Our general objective is to enable the application of vibrational spectroscopy to cancer diagnosis and treatment monitoring. The detection of cancer at its earliest stages is crucial, for it greatly improves the likelihood of successful treatment. Traditional methods of diagnosis have relied on physical removal of a portion of tissue and microscopic assessment of morphology. The need for tissue removal reduces the area of tissue that can be sampled. A noninvasive technique would eliminate this problem. Furthermore, a noninvasive technique has the potential to allow treatment to begin during the same endoscopic procedure used for diagnosis and reduce other complications associated with tissue removal such as tissue handling and increased risk of infection to the patient. We will focus on developing Raman spectroscopy for detection of precancerous conditions in patients with Barrett's esophagus. Barrett's esophagus is a pathology in which the squamous epithelial lining is replaced by a specialized metaplastic epithelium and the likelihood of adenocarcinoma is increased. Because the microscopic changes of dysplasia are difficult to observe, the entire area of metaplastic epithelium should be sampled. Therefore, Barrett's esophagus is well-suited for a noninvasive diagnostic technique. The methods and techniques developed in this proposal may also find application in other tissues such as the cervix. The second goal of our work is to develop Raman spectroscopy as a method for assessing the effects of treatment. Current methods for monitoring the response of an individual tumor to therapy are unreliable and often difficult to implement during the course of therapy. Development of noninvasive or minimally-invasive optical methods to reliably identify regions of apoptosis and necrosis would provide a simple method for assaying tumor response in each individual cancer patient. Consequently, treatments could be customized.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
6R01CA089255-06
Application #
7278914
Study Section
Diagnostic Imaging Study Section (DMG)
Program Officer
Nordstrom, Robert J
Project Start
2001-05-08
Project End
2008-02-28
Budget Start
2006-11-20
Budget End
2008-02-28
Support Year
6
Fiscal Year
2005
Total Cost
$403,278
Indirect Cost
Name
Los Alamos National Lab
Department
Type
DUNS #
175252894
City
Los Alamos
State
NM
Country
United States
Zip Code
87545
Kunapareddy, Nagapratima; Freyer, James P; Mourant, Judith R (2008) Raman spectroscopic characterization of necrotic cell death. J Biomed Opt 13:054002
Mourant, Judith R; Dominguez, Jorge; Carpenter, Susan et al. (2006) Comparison of vibrational spectroscopy to biochemical and flow cytometry methods for analysis of the basic biochemical composition of mammalian cells. J Biomed Opt 11:064024
Short, Kurt W; Carpenter, Susan; Freyer, James P et al. (2005) Raman spectroscopy detects biochemical changes due to proliferation in mammalian cell cultures. Biophys J 88:4274-88
Jiang, Yi; Pjesivac-Grbovic, Jelena; Cantrell, Charles et al. (2005) A multiscale model for avascular tumor growth. Biophys J 89:3884-94
Mourant, Judith R; Short, Kurt W; Carpenter, Susan et al. (2005) Biochemical differences in tumorigenic and nontumorigenic cells measured by Raman and infrared spectroscopy. J Biomed Opt 10:031106
Mourant, J R; Yamada, Y R; Carpenter, S et al. (2003) FTIR spectroscopy demonstrates biochemical differences in mammalian cell cultures at different growth stages. Biophys J 85:1938-47
Mourant, J R; Johnson, T M; Carpenter, S et al. (2002) Polarized angular dependent spectroscopy of epithelial cells and epithelial cell nuclei to determine the size scale of scattering structures. J Biomed Opt 7:378-87