Platinum anticancer drugs are currently the most widely used chemotherapeutic agents in medicine. The biological activity of platinum based anticancer drugs may, in part, be explained by differences in the mode of drug binding with DNA. A novel trinuclear platinum compound (BBR3464), has recently entered phase II clinical trials. This genuinely new platinum agent, not based on the cisplatin structure, represents a new class of antitumor drugs for which the mechanism of interaction with DNA, while clearly different than of cisplatin, remains poorly understood. The ain of this research is to probe, in real time, the kinetics of binding of a novel class of high-affinity polynuclear platinum compounds with un- labeled single and double stranded DNA oligomers immobilized on a surface plasmon resonance (SPR) sensor surface. This work will compare the modes of interaction for polynuclear and mononuclear platinum compounds, including cisplatin, with DNA. This work is distinguished of kinetics at interfaces. In addition, in situ temperature dependent SPR will be used for in-situ melting (dehybridization) studies with and without drug binding. The contribution of electrostatic interactions to drug binding with DNA oligomers will be determined by investigating the effect of varying solution ionic strength and by novel combination of SPR with electrochemical methods which will selectively alter the excess surface charge at the sensor interface. Finally, MALDI MS will be used to supplement our in-situ SPR kinetics measurements with addition mass spectrometric characterization of the platinated drug DNA adducts. This work provide a deeper understanding of the fundamental differences between novel multinuclear platinum drugs and the more well-studied cisplatin regarding the preferred modes of interaction with DNA. This information may offer insight on the differences in activity between mono- nuclear platinum compounds in vivo.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA089562-04
Application #
6752883
Study Section
Special Emphasis Panel (ZRG1-BECM (01))
Program Officer
Lees, Robert G
Project Start
2001-07-01
Project End
2006-06-30
Budget Start
2004-07-01
Budget End
2006-06-30
Support Year
4
Fiscal Year
2004
Total Cost
$183,375
Indirect Cost
Name
Boston University
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
049435266
City
Boston
State
MA
Country
United States
Zip Code
02215
Wolf, Lauren K; Gao, Yang; Georgiadis, Rosina M (2007) Kinetic discrimination of sequence-specific DNA-drug binding measured by surface plasmon resonance imaging and comparison to solution-phase measurements. J Am Chem Soc 129:10503-11
Gao, Yang; Wolf, Lauren K; Georgiadis, Rosina M (2006) Secondary structure effects on DNA hybridization kinetics: a solution versus surface comparison. Nucleic Acids Res 34:3370-7
Wolf, Lauren K; Fullenkamp, Dominic E; Georgiadis, Rosina M (2005) Quantitative angle-resolved SPR imaging of DNA-DNA and DNA-Drug kinetics. J Am Chem Soc 127:17453-9
Wolf, Lauren K; Gao, Yang; Georgiadis, Rosina M (2004) Sequence-dependent DNA immobilization: specific versus nonspecific contributions. Langmuir 20:3357-61
Peterson, Alexander W; Wolf, Lauren K; Georgiadis, Rosina M (2002) Hybridization of mismatched or partially matched DNA at surfaces. J Am Chem Soc 124:14601-7