Synovial sarcoma is a soft tissue tumor of the joints that affects young adults. It is characterized by a chromosomal translocation t(X; 18;pl 1;ql 1) that juxtaposes the SYT gene on chromosome 18 and the SSX gene on the X chromosome, resulting in the fusion, SYT-SSX. In the cancer, SYT-SSX is thought to exert a dominant gain of function, while the wt SYT allele is significantly down regulated, leading to loss of SYT normal function. a-catenin is the mediator of the wnt pathway, an active program in development and cancer. We found that SYT associates with beta-catenin in the nucleus and appears to suppress its function, while SYT-SSX activates it. The objective of this proposal is to characterize the functional relationship between SYT and beta-catenin, and its deregulation by SYT-SSX, in adult tissue and development. In addition, we will identify the nuclear components involved in this process and their role in mediating SYT and SYT-SSX effects on a-catenin.
The aim of this research proposal is to help gain a clearer understanding of synovial sarcoma development.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA106481-03
Application #
7069547
Study Section
Cancer Molecular Pathobiology Study Section (CAMP)
Program Officer
Woodhouse, Elizabeth
Project Start
2004-06-01
Project End
2009-04-30
Budget Start
2006-06-01
Budget End
2007-04-30
Support Year
3
Fiscal Year
2006
Total Cost
$241,820
Indirect Cost
Name
Vanderbilt University Medical Center
Department
Anatomy/Cell Biology
Type
Schools of Medicine
DUNS #
004413456
City
Nashville
State
TN
Country
United States
Zip Code
37212
Barco, Roy; Garcia, Christina B; Eid, Josiane E (2009) The synovial sarcoma-associated SYT-SSX2 oncogene antagonizes the polycomb complex protein Bmi1. PLoS One 4:e5060
Chittezhath, Manesh; Frump, Andrea L; Jourquin, Jerome et al. (2008) The proto-oncoprotein SYT (SS18) controls ATP release and regulates cyst formation by polarized MDCK cells. Exp Cell Res 314:3551-62
Barco, Roy; Hunt, Laura B; Frump, Andrea L et al. (2007) The synovial sarcoma SYT-SSX2 oncogene remodels the cytoskeleton through activation of the ephrin pathway. Mol Biol Cell 18:4003-12
Pretto, D; Barco, R; Rivera, J et al. (2006) The synovial sarcoma translocation protein SYT-SSX2 recruits beta-catenin to the nucleus and associates with it in an active complex. Oncogene 25:3661-9