? The goal of the proposed research is to develop, test, and validate a preclinical non-invasive optical instrument, based on spectroscopic Oblique Incidence Reflectometry (OIR), for the rapid diagnosis of pre-cancerous and invasive cancerous skin lesions. The underlying hypothesis, which is well supported by our strong preliminary clinical studies, is that quantitative, spatially resolved, optical-spectral (spatio-spectral) images of skin lesions can provide the dermatologist with clinically valuable diagnostic information to supplement his/her clinical judgment about the need for further diagnostic procedures and/or treatments. OIR intentionally breaks the symmetry of diffuse reflectance that exists in its normal-incidence counterpart and, as a result, provides more robust information about the interrogated tissue volume [Wang et al, US Patent, 5,630,423 (1997)]. A preliminary clinical study of 102 skin lesions using a handheld fiber-optic probe showed that the OIR spectroscopic system differentiated cancerous/pre-cancerous skin lesions from benign lesions with better than 95% accuracy. The applicants have identified the key diagnostic physiological parameters to be (1) the oxygen saturation of hemoglobin, (2) the concentration of total hemoglobin, and (3) the cell-nuclear size. The engineering goal of this proposal is to improve the prototype OIR system to measure skin lesions in real-time and process the data optimally for accurate lesion classification. The mechanistic goal is to identify and quantify the tissue and cellular factors that are responsible for the diagnostic optical features. The clinical goal is to provide accurate, objective, and real-time diagnoses for benign, pre-cancerous, and malignant lesions that are difficult to diagnose clinically. The ultimate societal impact is to improve patient care, save lives, and reduce health care costs.
The Specific Aims are listed as follows. 1. To improve instrumentation. 2. To establish a statistically significant database. 3. To develop and validate a diagnostic algorithm. 4. To identify the pathophysiologic parameters responsible for the diagnostic optical features. ? ?

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
5R01CA106728-02
Application #
6948509
Study Section
Diagnostic Imaging Study Section (DMG)
Program Officer
Liddell Huppi, Rebecca
Project Start
2004-09-15
Project End
2009-06-30
Budget Start
2005-09-01
Budget End
2006-06-30
Support Year
2
Fiscal Year
2005
Total Cost
$376,210
Indirect Cost
Name
Texas Engineering Experiment Station
Department
Biomedical Engineering
Type
Schools of Engineering
DUNS #
847205572
City
College Station
State
TX
Country
United States
Zip Code
77845
Garcia-Uribe, Alejandro; Zou, Jun; Duvic, Madeleine et al. (2012) In vivo diagnosis of melanoma and nonmelanoma skin cancer using oblique incidence diffuse reflectance spectrometry. Cancer Res 72:2738-45
Garcia-Uribe, A; Chang, C-C; Yapici, M K et al. (2011) High-Transmission-Efficiency and Side-Viewing Micro OIDRS Probe for Fast and Minimally-Invasive Tumor Margin Detection. IEEE Sens J 11:891-896
Garcia-Uribe, Alejandro; Smith, Elizabeth B; Zou, Jun et al. (2011) In-vivo characterization of optical properties of pigmented skin lesions including melanoma using oblique incidence diffuse reflectance spectrometry. J Biomed Opt 16:020501
Garcia-Uribe, A; Balareddy, K C; Zou, J et al. (2009) Micromachined ""Side-Viewing"" Optical Sensor Probe for Detection of Esophageal Cancers. Sens Actuators A Phys 150:144-150
Garcia-Uribe, Alejandro; Balareddy, Karthik C; Chang, Cheng-Chung et al. (2009) High-efficiency and side-viewing micro fiber optic probe for in-vivo diffuse reflectance measurements of human epithelial tissues. Conf Proc IEEE Eng Med Biol Soc 2009:757-60
Kothapalli, Sri-Rajasekhar; Wang, Lihong V (2009) Ex vivo blood vessel imaging using ultrasound-modulated optical microscopy. J Biomed Opt 14:014015
Kothapalli, Sri-Rajasekhar; Wang, Lihong V (2008) Ultrasound-modulated optical microscopy. J Biomed Opt 13:054046
Kim, Chulhong; Song, Kwang Hyun; Wang, Lihong V (2008) Sentinel lymph node detection ex vivo using ultrasound-modulated optical tomography. J Biomed Opt 13:020507
Kim, Chulhong; Wang, Lihong V (2007) Multi-optical-wavelength ultrasound-modulated optical tomography: a phantom study. Opt Lett 32:2285-7
Hollmann, Joseph L; Wang, Lihong V (2007) Multiple-source optical diffusion approximation for a multilayer scattering medium. Appl Opt 46:6004-9

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