Under past support, we have demonstrated the physics,mathematics, and instrumentation for near-infrared fluorescence enhanced (tomographic) optical imaging through centimeters of tissue-mimicking phantoms. In this competitive renewal we seek to translate fluorescence enhanced optical imaging for clinical cancer diagnostic imaging for sentinel lymph node mapping in patients. Herein, we gather a multidisciplinary team from Photon Migration Laboratory at Texas A&M University;The University of Texas M.D. Anderson Cancer Center, the University of Arizona's Center for Gamma Ray Imaging and the University of Texas at Austin's Institute for Computational Engineering and Science in order to: (1) improve upon existing frequency-domain photon migration (FDPM) imaging systems by reducing the noise floor and improving upon accuracy and precision of measurements required for clinical tomographic measurements using low-dose non-specific indocyanine green (ICG) and a molecularly targeting NIR dye; (2) utilize novel """"""""state-of-the-art"""""""" adaptive finite element methods in forward and inverse imaging solutions in order to improve the resolution of reconstructed images showing the depth of location and small size of sentinel lymph nodes and molecularly targeted, positive lymph nodes in breast cancer patients; (3) implement diagnostic cancer imaging using a developed clinical scanner with ICG or targeting NIR dyes in order to perform 2-D optical lymphography for comparison to nuclear lymphoscintigraphy and to perform 3-D tomography for determining the depth of sentinel nodes using adaptive finite element based tomographic algorithms and (4) implement diagnostic cancer imaging using the developed scanner with NIR dyes conjugated with the epidermal growth factor peptide as well as with a radiotracer for reporting positive lymph nodes of cancer patients with primary breast tumors which express the epidermal growth factor receptor in order to perform 2-D optical lymphography and 3-D tomography for reporting of positive nodes;and (5) apply image objective assessment of imaging quality tools to drive hardware and software improvements as well as to evaluate measurements and tomographic reconstructions. If successful, this project will provide the first clinical demonstration of fluorescence enhanced optical imaging in human patients with molecularly targeting NIR excitable contrast agents.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
3R01CA112679-14S1
Application #
7799984
Study Section
Biomedical Imaging Technology Study Section (BMIT)
Program Officer
Baker, Houston
Project Start
2005-09-29
Project End
2010-06-30
Budget Start
2008-10-01
Budget End
2009-06-30
Support Year
14
Fiscal Year
2009
Total Cost
$34,500
Indirect Cost
Name
University of Texas Health Science Center Houston
Department
Type
Schools of Medicine
DUNS #
800771594
City
Houston
State
TX
Country
United States
Zip Code
77225
Lu, Yujie; Darne, Chinmay D; Tan, I-Chih et al. (2015) Experimental Comparison of Continuous-Wave and Frequency-Domain Fluorescence Tomography in a Commercial Multi-Modal Scanner. IEEE Trans Med Imaging 34:1197-211
Rasmussen, John C; Aldrich, Melissa B; Guilliod, Renie et al. (2015) Near-infrared fluorescence lymphatic imaging in a patient treated for venous occlusion. J Vasc Surg Cases 1:201-204
Zhu, Banghe; Rasmussen, John C; Sevick-Muraca, Eva M (2014) A matter of collection and detection for intraoperative and noninvasive near-infrared fluorescence molecular imaging: to see or not to see? Med Phys 41:022105
Zhu, B; Tan, I-C; Rasmussen, J C et al. (2012) Validating the sensitivity and performance of near-infrared fluorescence imaging and tomography devices using a novel solid phantom and measurement approach. Technol Cancer Res Treat 11:95-104
Davies-Venn, Cynthia A; Angermiller, Bonnie; Wilganowski, Nathaniel et al. (2012) Albumin-binding domain conjugate for near-infrared fluorescence lymphatic imaging. Mol Imaging Biol 14:301-14
Hall, Mary A; Aldrich, Melissa B; Azhdarinia, Ali et al. (2012) Quantifying multimodal contrast agent biological activity using near-infrared flow cytometry. Contrast Media Mol Imaging 7:338-45
Rasmussen, John C; Tan, I-Chih; Marshall, Milton V et al. (2009) Lymphatic imaging in humans with near-infrared fluorescence. Curr Opin Biotechnol 20:74-82
Lee, Jae Hoon; Joshi, Amit; Sevick-Muraca, Eva M (2008) Fast intersections on nested tetrahedrons (FINT): An algorithm for adaptive finite element based distributed parameter estimation. J Comput Phys 227:5778-5798
Sevick-Muraca, Eva M; Sharma, Ruchi; Rasmussen, John C et al. (2008) Imaging of lymph flow in breast cancer patients after microdose administration of a near-infrared fluorophore: feasibility study. Radiology 246:734-41
Sampath, Lakshmi; Wang, Wei; Sevick-Muraca, Eva M (2008) Near infrared fluorescent optical imaging for nodal staging. J Biomed Opt 13:041312

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