The overall goal of this proposal is to elucidate the mechanisms by which lung tumor exosomes promote an inflammatory response, namely the activation of macrophages, and promote the progression and growth of lung tumors. Our preliminary data indicate that pretreatment of A/J mice with exosomes produced by TC-1 lung tumor cells or exosomes isolated from the lung tissue of NJ mice that have been pretreated with the carcinogen urethane results in more rapid tumor growth and earlier progression of lung carcinomas. Urethane treatment results in the recruitment of the activated form of TRAF2 to the exosomes, and the exosomes with activated TRAF2 are taken up by the bone marrow-derived precursors of macrophages, leading to their maturation and subsequent migration to the lung. The recruitment of the activated TRAF2 to the exosomes requires degradation of the inflammation suppressor protein, CYLD, in the tumor cells;moreover, TRAF2 can mediate ubiquitination of CYLD thereby promoting its degradation. The results of siRNA TRAF2 knockout indicated, however, that TRAF2 is required but is not sufficient for ubiquitination of CYLD. We have now identified two exosomal proteins that interact with TRAF2, Itch and Jabi, which have the potential to enhance the ubiquitination of CYLD in lung tumor cells. We propose to: (1) Confirm the role of lung tumor exosomal TRAF2 in the promotion of urethane-induced lung carcinomas by determining whether knockout of TRAF2 in TC-1 lung tumor cells is sufficient for (0 inhibition of macrophage differentiation and (ii) prevention of TC-1 cell exosome-mediated enhancement of urethane induced lung cancer. (2) Determine if Jabi and itch in the TC-1 exosomes are capable of promoting the differentiation of CD1 1bGr1 cells into activated macrophages. (3) Identification of cells targeted in vivo by exosomes. The data generated should permit the development a highly innovative model of the cellular and molecular basis for exosome-mediated chronic inflammation and promotion of lung tumor growth and suggest novel preventive and therapeutic strategies.

Public Health Relevance

Lung cancer is the leading cause of cancer-related death in the United States. Chronic inflammation plays a causative role in lung cancer. Therefore; the finding that exosomes isolated from lung tissues of mouse lung cancer model play a role in induction and subsequently activation of macrophages as proposed in this grant has several implications in the field of chronic inflammation related disease research including cancer; obesity; rheumatoid arthritis; atherosclerosis; and chronic obstructive pulmonary diseases. First; it suggests a central mechanism that regulates an important mode of cell-cell communication which may be of importance in communication among normal cells as well as several types of chronic inflammation driven diseases. The results of the experiments proposed in this application should indicate whether this mechanism is tumor-specific or not. Second; the identification of this mechanism provides a tool that can be used to clearly define the functions of carcinogen induced exosomes in the promotion of tumor growth; which has proven difficult to assign unambiguously. This will be addressed in this application for the analysis of the role of carcinogen (urethane) induced lung tumor exosomes in the activation of macrophages. Finally; the establishment of role of exosames CYLD cross-talked with TRAF2 in regulation of induction of activated macrophages will be of central importance as both molecules has long been recognized as a intracellular regulator of pro- and anti- inflammation proteins. But their role in intercellular communication has not been studied. This finding may also pinpoint novel molecular targets for the development of novel therapeutic strategies for the treatment of patients with chronic inflammation driven diseases like lung cancer. A finding that TRAF2 alone; or with other proteins; in the exosomes; is associated with the promotion of tumor growth may suggest a non-invasive procedure that will assist in prognosis of human lung cancer.

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
7R01CA137037-02
Application #
8015491
Study Section
Tumor Microenvironment Study Section (TME)
Program Officer
Howcroft, Thomas K
Project Start
2009-08-01
Project End
2011-07-31
Budget Start
2010-04-12
Budget End
2010-07-31
Support Year
2
Fiscal Year
2009
Total Cost
$307,876
Indirect Cost
Name
University of Louisville
Department
Microbiology/Immun/Virology
Type
Schools of Medicine
DUNS #
057588857
City
Louisville
State
KY
Country
United States
Zip Code
40292
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