Abstract

Multi-phase Microfluidic Devices for Characterization of Circulating Tumor Cells in Breast Cancer Oncogenesis is a multi-step process leading to a variety of genetic changes with alterations in the transcriptome, and adaptive changes responding to treatments. Therefore, monitoring the dynamic alteration of cancer is critical for cancer treatment. We have integrated multiple microfluidic modules developed in our R21 into a Multi-Phase Single Cell Analyzer (MSCA) for circulating tumor cells (CTC) enumeration and single CTC characterization. Combining the robustness of microstructure, preciseness of optical manipulation and mobility of droplet, the integrated MSCA isolates and encapsulates individual CTC into droplets for nanoliter molecular analysis. With the ability to characterize CTC in routine blood draws, the integrated platform will transform the minimal invasive CTC assessment into a powerful tool for cancer treatment evaluation in hospitals and clinical laboratories, and realize personalized medicine for cancer treatment.

Public Health Relevance

Combining the robustness of microstructure, mobility of droplet and preciseness of optical manipulation, the integrated MSCA can isolate and encapsulate individual CTCs into droplets for nanoliter molecular analysis. The MSCA device holds tremendous potential for new biological insight with very real clinical applications. The integrated platform will transform the minimal invasive CTC assessment into a powerful tool for cancer treatment evaluation in hospitals and clinical laboratories, and realize personalized medicine for cancer treatment.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Cancer Institute (NCI)
Type
Research Project (R01)
Project #
4R01CA164509-05
Application #
9057469
Study Section
Enabling Bioanalytical and Imaging Technologies Study Section (EBIT)
Program Officer
Ossandon, Miguel
Project Start
2012-05-01
Project End
2017-04-30
Budget Start
2016-05-01
Budget End
2017-04-30
Support Year
5
Fiscal Year
2016
Total Cost
Indirect Cost

  Institution

Name
University of Southern California
Department
Pathology
Type
Schools of Medicine
DUNS #
072933393
City
Los Angeles
State
CA
Country
United States
Zip Code
90032

  Publications

Li, Shengwen Calvin; Stucky, Andres; Chen, Xuelian et al. (2018) Single-cell transcriptomes reveal the mechanism for a breast cancer prognostic gene panel. Oncotarget 9:33290-33301
Jiao, Xuanmao; Velasco-Velázquez, Marco A; Wang, Min et al. (2018) CCR5 Governs DNA Damage Repair and Breast Cancer Stem Cell Expansion. Cancer Res 78:1657-1671
Chen, Xuelian; Wen, Qin; Stucky, Andres et al. (2018) Relapse pathway of glioblastoma revealed by single-cell molecular analysis. Carcinogenesis 39:931-936
Zeng, Yunjing; Gao, Li; Luo, Xiaoqing et al. (2018) Microfluidic enrichment of plasma cells improves treatment of multiple myeloma. Mol Oncol 12:1004-1011
Chen, Yan; Millstein, Joshua; Liu, Yao et al. (2018) Single-Cell Digital Lysates Generated by Phase-Switch Microfluidic Device Reveal Transcriptome Perturbation of Cell Cycle. ACS Nano 12:4687-4694
Stucky, Andres; Chen, Xuelian; Zhong, Jiang F (2018) Gene Manipulation with Micro RNAs at Single-Human Cancer Cell. Methods Mol Biol 1733:215-223
Li, Shengwen Calvin; Vu, Long T; Luo, Jane Jianying et al. (2017) Tissue Elasticity Bridges Cancer Stem Cells to the Tumor Microenvironment Through microRNAs: Implications for a ""Watch-and-Wait"" Approach to Cancer. Curr Stem Cell Res Ther 12:455-470
Liu, Jun; Zhong, Jiang F; Zhang, Xi et al. (2017) Allogeneic CD19-CAR-T cell infusion after allogeneic hematopoietic stem cell transplantation in B cell malignancies. J Hematol Oncol 10:35
Zhang, Cheng; Yang, Shi-Jie; Wen, Qin et al. (2017) Human-derived normal mesenchymal stem/stromal cells in anticancer therapies. J Cancer 8:85-96
Chen, Xin; Chakravarty, Tushar; Zhang, Yiqiang et al. (2016) Single-cell transcriptome and epigenomic reprogramming of cardiomyocyte-derived cardiac progenitor cells. Sci Data 3:160079

Showing the most recent 10 out of 17 publications