Abstract

A research program dealing with the biochemistry and pharmacology of endogenous opioid peptides. Particular attention is given to the formation, metabolic inactivation, and binding sites for those materials. The work has five foci: covalent modifications of endogenous peptides; effects of narcotic administration on regulation of peptide synthesis; enkephalinase as an enzyme involved in the inactivation of opioid peptides; multiple opiate receptors in brain; and effects of morphine administration during pregnancy on maternal-fetal peptide systems. In each area, investigations using administration, withdrawal, and addiction to opioids are central. Through such studies we would hope to learn more about basic mechanisms related to morphine use and abuse.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute on Drug Abuse (NIDA)
Type
Research Project (R01)
Project #
5R01DA001207-12
Application #
3206884
Study Section
(SRC)
Project Start
1975-06-27
Project End
1988-08-31
Budget Start
1986-09-30
Budget End
1987-08-31
Support Year
12
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
Stanford University
Department
Type
Schools of Medicine
DUNS #
800771545
City
Stanford
State
CA
Country
United States
Zip Code
94305

  Publications

Weitz, C J; Lowney, L I; Faull, K F et al. (1988) 6-Acetylmorphine: a natural product present in mammalian brain. Proc Natl Acad Sci U S A 85:5335-8
Hurlbut, D E; Evans, C J; Barchas, J D et al. (1987) Pharmacological properties of a proenkephalin A-derived opioid peptide: BAM 18. Eur J Pharmacol 138:359-66
Eberwine, J H; Barchas, J D; Hewlett, W A et al. (1987) Isolation of enzyme cDNA clones by enzyme immunodetection assay: isolation of a peptide acetyltransferase. Proc Natl Acad Sci U S A 84:1449-53
Roth, K A; Bensch, K G; Hoffman, A R (1987) Characterization of opioid peptides in human thyroid medullary carcinoma. Cancer 59:1594-8
Beck, O; Tyler, A; Faull, K (1987) Serotonin condensation product 5-hydroxymethtryptoline: evidence for in vivo formation from acetaldehyde during intoxication using deuterium labelled ethanol. Alcohol Alcohol Suppl 1:743-7
Faull, K F (1987) Application of combined gas chromatography/mass spectrometry to neurotransmitter research. Life Sci 41:889-92
Lorenz, R G; Tyler, A N; Faull, K F et al. (1986) Characterization of endorphins from the pituitary of the spiny dogfish Squalus acanthias. Peptides 7:119-26
Roth, K A; Barchas, J D (1986) Small cell carcinoma cell lines contain opioid peptides and receptors. Cancer 57:769-73
Weitz, C J; Lowney, L I; Faull, K F et al. (1986) Morphine and codeine from mammalian brain. Proc Natl Acad Sci U S A 83:9784-8
Evans, C J; Barchas, J D; Esch, F S et al. (1985) Isolation and characterization of an endogenous C-terminal fragment of the alpha-neo-endorphin/dynorphin precursor from bovine caudate nucleus. J Neurosci 5:1803-7

Showing the most recent 10 out of 11 publications