Abstract

The objective of the project is to understand the mechanism of action of opiate and opioid peptides at the biochemical and physiological level by making use of neurotumor clonal cell lines which express different types of opiate receptors. The goals for the coming year are to continue studies on the cellular changes which result from acute or chronic exposure to opiates, specifically focussing on adenylate cyclase, protein kinase and glycosyltransferase linkage, and changes in ion fluxes and membrane potential. For these studies we will use cell lines N4TG1 and NG108-15 which express only DELTA (enkephalin) receptors, NCB-20, which express two BENZOMORPHAN (KAPPA AND SIGMA) receptors in addition to DELTA, and TCX-17 (which may express MU (MORPHINE) receptors in addition to DELTA receptors.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute on Drug Abuse (NIDA)
Type
Research Project (R01)
Project #
5R01DA002575-06
Application #
3207419
Study Section
(SRC)
Project Start
1980-04-01
Project End
1986-03-31
Budget Start
1985-04-01
Budget End
1986-03-31
Support Year
6
Fiscal Year
1985
Total Cost
Indirect Cost

  Institution

Name
University of Chicago
Department
Type
Schools of Medicine
DUNS #
225410919
City
Chicago
State
IL
Country
United States
Zip Code
60637

  Publications

Sun, L; Miller, R J (1999) Multiple neuropeptide Y receptors regulate K+ and Ca2+ channels in acutely isolated neurons from the rat arcuate nucleus. J Neurophysiol 81:1391-403
Fatatis, A; Miller, R J (1999) Cell cycle control of PDGF-induced Ca(2+) signaling through modulation of sphingolipid metabolism. FASEB J 13:1291-301
Ford, C E; Skiba, N P; Bae, H et al. (1998) Molecular basis for interactions of G protein betagamma subunits with effectors. Science 280:1271-4
Jordan, J; Galindo, M F; Miller, R J et al. (1998) Isoform-specific effect of apolipoprotein E on cell survival and beta-amyloid-induced toxicity in rat hippocampal pyramidal neuronal cultures. J Neurosci 18:195-204
Simen, A A; Miller, R J (1998) Structural features determining differential receptor regulation of neuronal Ca channels. J Neurosci 18:3689-98
Lee, C C; Miller, R J (1998) Is there really an NPY Y3 receptor? Regul Pept 75-76:71-8
Bindokas, V P; Lee, C C; Colmers, W F et al. (1998) Changes in mitochondrial function resulting from synaptic activity in the rat hippocampal slice. J Neurosci 18:4570-87
Kinney, G A; Emmerson, P J; Miller, R J (1998) Galanin receptor-mediated inhibition of glutamate release in the arcuate nucleus of the hypothalamus. J Neurosci 18:3489-500
Sun, L; Philipson, L H; Miller, R J (1998) Regulation of K+ and Ca++ channels by a family of neuropeptide Y receptors. J Pharmacol Exp Ther 284:625-32
Rhim, H; Kinney, G A; Emmerson, P J et al. (1997) Regulation of neurotransmission in the arcuate nucleus of the rat by different neuropeptide Y receptors. J Neurosci 17:2980-9

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