A continuation of the in vivo intracellular recording and labeling study of the dorsal (DCN) and posteroventral cochlear nuclei (PVCN) is proposed. After conducting a series of extracellular single-unit and two-electrode studies in both the barbiturate anesthetized and unanesthetized decerebrate gerbil, a database of response types for gerbil has been established. Two new, substantial physiological classes based on responses to noise have been discovered. Intracellular responses of cochlear nucleus projection neurons and interneurons to both acoustic stimulation and current- pulse injection through the recording electrode have been recorded. Identification of these neurons using neurobiotin as the cellular marker has resulted in an increase of recovered neurons compared to HRP. We need to continue this work in DCN and expand it to the PVCN, where we propose to identify the neurons that project form the PVCN to the DCN and elucidate their acoustic response properties. In order to understand the relationships between the response properties of units recorded in the decerebrate versus the barbiturate anesthetized preparation, we propose to study the effects of an ultrashort acting barbiturate on the acoustic responses of units recorded in the DCN of decerebrate preparations.

Agency
National Institute of Health (NIH)
Institute
National Institute on Deafness and Other Communication Disorders (NIDCD)
Type
Research Project (R01)
Project #
2R01DC001099-05A2
Application #
2395284
Study Section
Special Emphasis Panel (ZRG1-HAR (01))
Project Start
1991-04-01
Project End
2002-06-30
Budget Start
1997-07-01
Budget End
1998-06-30
Support Year
5
Fiscal Year
1997
Total Cost
Indirect Cost
Name
Boston University
Department
Biomedical Engineering
Type
Schools of Engineering
DUNS #
604483045
City
Boston
State
MA
Country
United States
Zip Code
02118
Navawongse, Rapeechai; Voigt, Herbert F (2009) Single neuron recordings in dorsal cochlear nucleus (DCN) of awake gerbil. Hear Res 255:44-57
Hancock, Kenneth E; Voigt, Herbert F (2002) Intracellularly labeled fusiform cells in dorsal cochlear nucleus of the gerbil. II. Comparison of physiology and anatomy. J Neurophysiol 87:2520-30
Hancock, Kenneth E; Voigt, Herbert F (2002) Intracellularly labeled fusiform cells in dorsal cochlear nucleus of the gerbil. I. Physiological response properties. J Neurophysiol 87:2505-19
Ding, J; Benson, T E; Voigt, H F (1999) Acoustic and current-pulse responses of identified neurons in the dorsal cochlear nucleus of unanesthetized, decerebrate gerbils. J Neurophysiol 82:3434-57
Davis, K A; Voigt, H F (1997) Evidence of stimulus-dependent correlated activity in the dorsal cochlear nucleus of decerebrate gerbils. J Neurophysiol 78:229-47
Ding, J; Voigt, H F (1997) Intracellular response properties of units in the dorsal cochlear nucleus of unanesthetized decerebrate gerbil. J Neurophysiol 77:2549-72
Gdowski, G T; Voigt, H F (1997) Response map properties of units in the dorsal cochlear nucleus of barbiturate-anesthetized gerbil (Meriones unguiculatus). Hear Res 105:85-104
Ravicz, M E; Rosowski, J J; Voigt, H F (1996) Sound-power collection by the auditory periphery of the mongolian gerbil Meriones unguiculatus. II. External-ear radiation impedance and power collection. J Acoust Soc Am 99:3044-63
Davis, K A; Ding, J; Benson, T E et al. (1996) Response properties of units in the dorsal cochlear nucleus of unanesthetized decerebrate gerbil. J Neurophysiol 75:1411-31
Davis, K A; Voigt, H F (1996) Computer simulation of shared input among projection neurons in the dorsal cochlear nucleus. Biol Cybern 74:413-25

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