Abstract

The principal investigator proposes to utilize Drosophila melanogaster in his studies of the components of the olfactory signal transduction pathways. Mutants generated by P-element insertion will be isolated and examined for phenotype, the sequence of the gene affected, reversion by P-element mutagenesis, tissue specificity of expression. The isolated gene will be expressed in the baculovirus system and if the gene resembles a receptor, the odorant-induced, second messengers will be examined in the Sf9 cells that are used to express the gene in baculovirus.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute on Deafness and Other Communication Disorders (NIDCD)
Type
Research Project (R01)
Project #
5R01DC002485-03
Application #
2014555
Study Section
Sensory Disorders and Language Study Section (CMS)
Project Start
1995-01-01
Project End
1998-06-30
Budget Start
1997-01-01
Budget End
1998-06-30
Support Year
3
Fiscal Year
1997
Total Cost
Indirect Cost

  Institution

Name
North Carolina State University Raleigh
Department
Zoology
Type
Schools of Earth Sciences/Natur
DUNS #
City
Raleigh
State
NC
Country
United States
Zip Code
27695

  Publications

Kulkarni, N H; Karavanich, C A; Atchley, W R et al. (2000) Characterization and differential expression of a human gene family of olfactomedin-related proteins. Genet Res 76:41-50
Wekesa, K S; Anholt, R R (1999) Differential expression of G proteins in the mouse olfactory system. Brain Res 837:117-26
Karavanich, C A; Anholt, R R (1998) Molecular evolution of olfactomedin. Mol Biol Evol 15:718-26
Fedorowicz, G M; Fry, J D; Anholt, R R et al. (1998) Epistatic interactions between smell-impaired loci in Drosophila melanogaster. Genetics 148:1885-91
Wekesa, K S; Anholt, R R (1997) Pheromone regulated production of inositol-(1, 4, 5)-trisphosphate in the mammalian vomeronasal organ. Endocrinology 138:3497-504
Mackay, T F; Hackett, J B; Lyman, R F et al. (1996) Quantitative genetic variation of odor-guided behavior in a natural population of Drosophila melanogaster. Genetics 144:727-35
Anholt, R R; Lyman, R F; Mackay, T F (1996) Effects of single P-element insertions on olfactory behavior in Drosophila melanogaster. Genetics 143:293-301