We propose to examine inner ear development using zebra fish, Danio rerio, as a model system. Zebra fish has emerged as a powerful experimental organism because of the ease of use in developmental biology and genetic studies. The zebra fish inner ear is essentially the same as that in other vertebrates: a closed epithelial structure with three communicating chambers and three semicircular canals. However, no cochlea or cochlear duct is found in the fish ear. Sensory patches are found within the chambers and canals, composed of hair cells and supporting cells. Hair cells synapse with statoacoustic ganglion (SAG) cells that relay the signals to the brain. Otoliths in the matrix above the sensory patches allow teleost fish to detect mechanical stimulation, including linear and rotational acceleration, gravity and sound. Cadherin cell adhesion molecules control a variety of developmental processes, including processes like cell sorting, differentiation and neurite formation in the nervous system. We propose to determine the role of cadherin cell adhesion molecules during the development of the inner ear and SAG.
Three specific aims will be employed; (1) determine expression pattems of cadherins in the inner ear of developing zebra fish; (2) determine the function of cadherins during inner ear development; and (3) determine the effects of Pax-2 gene function on cadherin expression during otic placode induction, inner ear development and statoacoustic ganglion (SAG) formation.
|Ramamurthy, Poornapriya; White, Joshua B; Yull Park, Joong et al. (2017) Concomitant differentiation of a population of mouse embryonic stem cells into neuron-like cells and schwann cell-like cells in a slow-flow microfluidic device. Dev Dyn 246:7-27|
|Song, Sungmin; Eckerle, Stephanie; Onichtchouk, Daria et al. (2013) Pou5f1-dependent EGF expression controls E-cadherin endocytosis, cell adhesion, and zebrafish epiboly movements. Dev Cell 24:486-501|
|Bank, Lisa M; Bianchi, Lynne M; Ebisu, Fumi et al. (2012) Macrophage migration inhibitory factor acts as a neurotrophin in the developing inner ear. Development 139:4666-74|
|Clendenon, Sherry G; Sarmah, Swapnalee; Shah, Bijal et al. (2012) Zebrafish cadherin-11 participates in retinal differentiation and retinotectal axon projection during visual system development. Dev Dyn 241:442-54|
|Clendenon, Sherry G; Shah, Bijal; Miller, Caroline A et al. (2009) Cadherin-11 controls otolith assembly: evidence for extracellular cadherin activity. Dev Dyn 238:1909-22|
|Liu, Qin; Marrs, James A; Londraville, Richard L et al. (2008) Cadherin-7 function in zebrafish development. Cell Tissue Res 334:37-45|
|Liu, Qin; Londraville, Richard; Marrs, James A et al. (2008) Cadherin-6 function in zebrafish retinal development. Dev Neurobiol 68:1107-22|
|Shen, Yu-Chi; Jeyabalan, Anandhi K; Wu, Karen L et al. (2008) The transmembrane inner ear (tmie) gene contributes to vestibular and lateral line development and function in the zebrafish (Danio rerio). Dev Dyn 237:941-52|
|Wilson, Amy L; Shen, Yu-Chi; Babb-Clendenon, S G et al. (2007) Cadherin-4 plays a role in the development of zebrafish cranial ganglia and lateral line system. Dev Dyn 236:893-902|
|Liu, Bei; Joel Duff, R; Londraville, Richard L et al. (2007) Cloning and expression analysis of cadherin7 in the central nervous system of the embryonic zebrafish. Gene Expr Patterns 7:15-22|
Showing the most recent 10 out of 13 publications