The basic synaptic organization of the olfactory bulb (OB) has been known for several decades, but we continue to lack insight into the properties of these synaptic circuits and their underlying molecular determinants. The structural and molecular properties of axodendritic (A:D) synapses have been studied elsewhere in brain, but little work has been done on the A:D and dendrodendritic (D:D) synapses in the OB. Indeed, since the first descriptions of these synapses in the early '70s, there have been few reports on their structural/molecular properties. This paucity of information conflicts with the growing interest in the physiology of A:D and D:D synapses in the OB and their mechanisms of regulation. The current proposal has 3 specific aims that will generate new insights into the architectural/molecular properties of D:D synapses.
In Aim 1 immunohistochemical techniques at both the confocal and electron microscopic levels will establish the distribution of synaptic vesicle associated proteins at A:D and D:D OB synapses. We hypothesize that dendritic architecture may lead to the expression of unique members of the vesicle cascade at D:D synapses.
In Aim 2 we propose to use 3D reconstructions to examine the morphometric features of these synapses and test the hypothesis that the characteristics of the synapses are correlated.
In Aim 3 we will examine critically the development of reciprocal A:D and D:D appositions in the OB at the ultrastructural level. Early studies suggested that the Gray Type 1 synapse may form first, followed by the Gray Type 2. However, this was based on single sections and morphometric statistics rather than the careful systematic 3D reconstructions that we propose. Similarly, while the role of functional activity in the development of the OB remains controversial, no studies have examined development at the ultrastructural level. In sum, we believe that these studies will provide significant new insights into the molecular mechanisms and vesicle proteins that regulate synaptic function at both A:D and D:D synapses in the OB. ? ?

Agency
National Institute of Health (NIH)
Institute
National Institute on Deafness and Other Communication Disorders (NIDCD)
Type
Research Project (R01)
Project #
1R01DC006972-01
Application #
6830542
Study Section
Special Emphasis Panel (ZRG1-IFCN-A (02))
Program Officer
Davis, Barry
Project Start
2004-07-22
Project End
2009-05-31
Budget Start
2004-07-22
Budget End
2005-05-31
Support Year
1
Fiscal Year
2004
Total Cost
$367,875
Indirect Cost
Name
Yale University
Department
Neurosurgery
Type
Schools of Medicine
DUNS #
043207562
City
New Haven
State
CT
Country
United States
Zip Code
06520
Rela, Lorena; Piantanida, Ana Paula; Bordey, Angelique et al. (2015) Voltage-dependent K+ currents contribute to heterogeneity of olfactory ensheathing cells. Glia 63:1646-59
Mobley, Arie S; Bryant, Alex K; Richard, Marion B et al. (2013) Age-dependent regional changes in the rostral migratory stream. Neurobiol Aging 34:1873-81
Rela, Lorena; Bordey, Angelique; Greer, Charles A (2010) Olfactory ensheathing cell membrane properties are shaped by connectivity. Glia 58:665-78
Richard, Marion B; Taylor, Seth R; Greer, Charles A (2010) Age-induced disruption of selective olfactory bulb synaptic circuits. Proc Natl Acad Sci U S A 107:15613-8
Rodriguez-Gil, Diego J; Treloar, Helen B; Zhang, Xiaohong et al. (2010) Chromosomal location-dependent nonstochastic onset of odor receptor expression. J Neurosci 30:10067-75
Rodriguez-Gil, Diego J; Treloar, Helen B; Miller, Alexandra et al. (2009) Onset of odorant receptors. Ann N Y Acad Sci 1170:18-20
Imamura, Fumiaki; Greer, Charles A (2009) Dendritic branching of olfactory bulb mitral and tufted cells: regulation by TrkB. PLoS One 4:e6729
Whitman, Mary C; Greer, Charles A (2009) Adult neurogenesis and the olfactory system. Prog Neurobiol 89:162-75
Whitman, Mary C; Fan, Wen; Rela, Lorena et al. (2009) Blood vessels form a migratory scaffold in the rostral migratory stream. J Comp Neurol 516:94-104
Rodriguez-Gil, Diego J; Greer, Charles A (2008) Wnt/Frizzled family members mediate olfactory sensory neuron axon extension. J Comp Neurol 511:301-17

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