The long-term objectives of this proposal are twofold: (1) to identify neurotransmitters released from rat and mouse taste bud cells during gustatory stimulation and (2) to investigate signal processing within the taste bud. These both are significant unanswered questions in the field of chemosensory reception. To date, there is only indirect evidence for candidate neurotransmitters in taste cells, including serotonin, glutamate, norepinephrine, acetylcholine, ATP, and peptides. We have developed a novel methodology to dentify the neurotransmitter(s) released from rat and mouse taste buds during gustatory stimulation. Our methodology uses Chinese Hamster Ovary (CHO) cells stably expressing high affinity receptors for candidate neurotransmitters and loaded with the calcium-sensitive dye, Fura 2. We will use these cells as biosensors to detect tastant-evoked release of neurotransmitters from isolated tasted buds and taste cells.
The specific aims i nclude investigating whether taste stimulation elicits release of serotonin, norepinephrine, acetylcholine, glutamate, ATP, CCK, and VIP from mourse and rat taste buds removed from vallate, foliate, palatal, and fungiform taste fields. Further, we aim to investigate whether such release is mediated by Ca2+ influx, intracellular Ca2+ release, or via transporter-mediated mechanisms. Finally, we propose to investigate cell-to-cell communication within taste buds via paracrine substances such as ATP and glutamate, and how information is transmitted from sensory receptor cells to synaptic output cells and on to primary sensory afferent terminals. The project will illuminate information processing in peripheral taste organs and may solve the conundrum of how signals generated in one cell (receptor cells) are transmitted to other cells (synaptic output cells) in the taste bud. The results may resolve the dilemma that taste cells appear to express only a specified set of taste receptors (i.e., bitter, or sweet, or umami GPCRs). Yet, some taste cells and many sensory afferent nerve fibers respond to multiple taste stimuli. The project will begin to raise our understanding of how taste signals are refined in the periphery, perhaps comparable to how visual signals are processed in the retina

Agency
National Institute of Health (NIH)
Institute
National Institute on Deafness and Other Communication Disorders (NIDCD)
Type
Research Project (R01)
Project #
5R01DC007630-03
Application #
7388793
Study Section
Somatosensory and Chemosensory Systems Study Section (SCS)
Program Officer
Davis, Barry
Project Start
2006-04-01
Project End
2011-03-31
Budget Start
2008-04-01
Budget End
2009-03-31
Support Year
3
Fiscal Year
2008
Total Cost
$311,594
Indirect Cost
Name
University of Miami School of Medicine
Department
Physiology
Type
Schools of Medicine
DUNS #
052780918
City
Coral Gables
State
FL
Country
United States
Zip Code
33146
Roper, Stephen D; Chaudhari, Nirupa (2017) Taste buds: cells, signals and synapses. Nat Rev Neurosci 18:485-497
Dando, Robin; Pereira, Elizabeth; Kurian, Mani et al. (2015) A permeability barrier surrounds taste buds in lingual epithelia. Am J Physiol Cell Physiol 308:C21-32
Meredith, Tricia L; Corcoran, Alan; Roper, Stephen D (2015) Leptin's effect on taste bud calcium responses and transmitter secretion. Chem Senses 40:217-22
Roper, Stephen D (2015) The taste of table salt. Pflugers Arch 467:457-63
Roper, Stephen D (2014) Sensory end-organs: signal processing in the periphery: a symposium presented at the 2013 Annual Meeting of the Society for Neuroscience, San Diego, CA, USA. J Physiol 592:3383-5
Roper, Stephen D (2014) TRPs in taste and chemesthesis. Handb Exp Pharmacol 223:827-71
Roper, Stephen D (2013) Introduction to signal processing in peripheral sensory organs. Semin Cell Dev Biol 24:1-2
Roper, Stephen D (2013) Taste buds as peripheral chemosensory processors. Semin Cell Dev Biol 24:71-9
Huang, Yijen A; Grant, Jeff; Roper, Stephen (2012) Glutamate may be an efferent transmitter that elicits inhibition in mouse taste buds. PLoS One 7:e30662
Dando, Robin; Roper, Stephen D (2012) Acetylcholine is released from taste cells, enhancing taste signalling. J Physiol 590:3009-17

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