Clinically significant hearing loss is present in at least 1.9 per 1,000 infants at birth and affects at least 30% of the population at some time in their lives. Inner ear anomalies, detected by a computerized scan or magnetic resonance imaging study, have been reported in up to one third of children with deafness. Despite recent progress in identifying genes that determine many forms of syndromic and non-syndromic sensorineural hearing loss, the genetic pathogenesis of inner ear anomalies has remained largely unknown. Most of the individuals with inner ear anomalies are the only affected members of their families (simplex) or come from small-sized families preventing the usage of traditional positional cloning strategies. Recent advances in sequencing technology have provided unprecedented opportunities for the large scale screening of DNA variation throughout individual genomes and opened the way of gene discovery in small-sized families or in simplex cases. We have successfully applied this technology to identification of responsible DNA changes in various Mendelian disorders. In this study we will use our existing Repository of participants with inner ear anomalies that currently contains biological samples and clinical data from 203 families. Parental consanguinity is present in 10 families, 3 of which are multiplex, providing strong evidence for autosomal recessive inheritance. DNA samples from families will undergo genotyping with genomewide SNP arrays to detect autozygous regions in families with parental consanguinity and to identify potential pathogenic copy number variants. Then, whole exome sequencing and bioinformatics analysis with an established exome analysis pipeline at the Hussman Institute for Human Genomics (HIHG) will be applied to find causative variants. Co- segregating homozygous variants in consanguineous families and de novo variants in simplex families will be further investigated. Sanger sequencing and TaqMan genotyping will serve to screen additional families, and to study variant allele frequencies in ethnicity-matched controls. To support the role of identified changes in pathophysiology, zebrafish models will be rapidly produced. Further support will be obtained from mouse models. The outcomes of this proposal will be discoveries of new genes/pathways involved in the development of the inner ear in humans and foundation of clinical molecular diagnostic tests for better diagnosis and counseling of deaf individuals with inner ear anomalies.

Public Health Relevance

The goals of the project are to identify new genes for deafness associated with inner ear anomalies and to establish a resource for research on inner ear anomalies including biological samples and clinical data from large numbers of affected families. The outcome of this proposal will expand the scientific knowledge on the genomic basis of inner ear anomalies and provide molecular diagnostic tools to the affected families.

Agency
National Institute of Health (NIH)
Institute
National Institute on Deafness and Other Communication Disorders (NIDCD)
Type
Research Project (R01)
Project #
5R01DC012836-05
Application #
9206152
Study Section
Genetics of Health and Disease Study Section (GHD)
Program Officer
Watson, Bracie
Project Start
2013-03-01
Project End
2019-02-28
Budget Start
2017-03-01
Budget End
2019-02-28
Support Year
5
Fiscal Year
2017
Total Cost
Indirect Cost
Name
University of Miami School of Medicine
Department
Genetics
Type
Schools of Medicine
DUNS #
052780918
City
Coral Gables
State
FL
Country
United States
Zip Code
33146
Booth, K T; Kahrizi, K; Babanejad, M et al. (2018) Variants in CIB2 cause DFNB48 and not USH1J. Clin Genet 93:812-821
Cengiz, Filiz Basak; Yilmazer, Rasim; Olgun, Levent et al. (2017) Novel pathogenic variants underlie SLC26A4-related hearing loss in a multiethnic cohort. Int J Pediatr Otorhinolaryngol 101:167-171
Suba??o?lu, Asl?; Duman, Duygu; S?rmac?, Asl? et al. (2017) Research of genetic bases of hereditary non-syndromic hearing loss. Turk Pediatri Ars 52:122-132
Klingbeil, Kyle D; Greenland, Christopher M; Arslan, Selcuk et al. (2017) Novel EYA1 variants causing Branchio-oto-renal syndrome. Int J Pediatr Otorhinolaryngol 98:59-63
Tekin, Demet; Yan, Denise; Bademci, Guney et al. (2016) A next-generation sequencing gene panel (MiamiOtoGenes) for comprehensive analysis of deafness genes. Hear Res 333:179-184
Diaz-Horta, Oscar; Abad, Clemer; Sennaroglu, Levent et al. (2016) ROR1 is essential for proper innervation of auditory hair cells and hearing in humans and mice. Proc Natl Acad Sci U S A 113:5993-8
Yao, Qi; DeSmidt, Alexandra A; Tekin, Mustafa et al. (2016) Hearing Assessment in Zebrafish During the First Week Postfertilization. Zebrafish 13:79-86
Bademci, G; Cengiz, F B; Foster Ii, J et al. (2016) Variations in Multiple Syndromic Deafness Genes Mimic Non-syndromic Hearing Loss. Sci Rep 6:31622
Yan, Denise; Tekin, Demet; Bademci, Guney et al. (2016) Spectrum of DNA variants for non-syndromic deafness in a large cohort from multiple continents. Hum Genet 135:953-61
Manzoli, Gabrielle N; Bademci, Guney; Acosta, Angelina X et al. (2016) Targeted Resequencing of Deafness Genes Reveals a Founder MYO15A Variant in Northeastern Brazil. Ann Hum Genet 80:327-331

Showing the most recent 10 out of 12 publications