We have been studying the basic biochemical mechanisms whereby the type of dietary lipids can affect the salivary gland cell function. The working hypothesis being investigated is that dietary lipids can induce changes in structural lipids and fluidity of the cell membranes, which results in modification of membrane function. In vivo changes can be induced in membrane lipids by essential fatty acid deficiency, cholesterol-rich diets and diets containing high levels of omega-3 fatty acids. Changes in membrane lipids result in altering beta-receptor adenylate cyclase system, which consists of the receptor, G-proteins and adenylate cyclase. Further studies will be conducted using these nutritional models to determine their effects on each of the 3 components of the adenylate cyclase system in the rat submandibular salivary gland (SMSG). Studies will also be conducted with dispersed SMSG cells from rats fed various diets in order to determine if the diet-induced changes in membrane lipids and adenylate cyclase system would result in. functional changes such as the secretion of glycoproteins. Diet-induced changes in membrane fluidity will be assessed by measuring fluorescence polarization and lifetimes of the excited states for diphenylhexatriene and 16-anthroyloxy palmitate after their incorporation into the SMSG membranes. Because of the microsomal fatty acid desaturases are considered to be important in the regulation of membrane fluidity and in the metabolism of essential fatty acids, their specific activities in the SMSG, along with their dietary modulation, will be studied.

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Research Project (R01)
Project #
5R01DE005978-08
Application #
3219696
Study Section
Oral Biology and Medicine Subcommittee 1 (OBM)
Project Start
1983-09-01
Project End
1994-02-28
Budget Start
1992-03-01
Budget End
1993-02-28
Support Year
8
Fiscal Year
1992
Total Cost
Indirect Cost
Name
Louisiana State University Hsc New Orleans
Department
Type
Schools of Dentistry
DUNS #
782627814
City
New Orleans
State
LA
Country
United States
Zip Code
70112
Alam, S Q; Shi, Y Y (1997) The effect of essential fatty acid deficiency on the fatty acid composition of different salivary glands and saliva in rats. Arch Oral Biol 42:727-34
Alam, S Q; Abdel-Hakim, S M; Alam, B S et al. (1995) Effect of essential fatty acid deficiency on G-proteins, cAMP-dependent protein kinase activity and mucin secretion in the rat submandibular salivary glands. Cell Signal 7:773-81
Abdel-Hakim, S M; Alam, B S; Alam, S Q (1994) Prostaglandin E2 production in submandibular salivary glands of rats in essential fatty acid deficiency. Prostaglandins Leukot Essent Fatty Acids 50:141-5
Alam, S Q; Mannino, S J; Alam, B S (1993) Reversal of diet-induced changes in adenylate cyclase activity and fatty acid composition of rat submandibular salivary gland lipids. Arch Oral Biol 38:387-91
Alam, S Q; Bergens, B M; Alam, B S (1991) Arachidonic acid, prostaglandin E2 and leukotriene C4 levels in gingiva and submandibular salivary glands of rats fed diets containing n-3 fatty acids. Lipids 26:895-900
Ahmad, S N; Alam, S Q; Alam, B S (1990) Influence of dietary omega-3 fatty acids on transmembrane signalling in rat submandibular salivary gland. Cell Signal 2:29-41
Ahmad, S N; Alam, S Q; Alam, B S (1990) Effect of ageing on adenylate cyclase activity and G-proteins in rat submandibular salivary glands. Arch Oral Biol 35:885-90
Ahmad, S N; Alam, S Q; Alam, B S (1990) Fatty acid incorporation into membranes of dispersed rat submandibular salivary gland cells and their effect on adenylate cyclase activity. Arch Oral Biol 35:879-83
Alam, S Q; Alam, B S (1988) In-vivo incorporation of omega 3 fatty acids into membrane lipids of rat salivary glands and changes in adenylate-cyclase activity. Arch Oral Biol 33:295-9
Ren, Y F; Alam, S Q; Alam, B S et al. (1988) Adenylate cyclase and beta-receptors in salivary glands of rats fed diets containing trans fatty acids. Lipids 23:304-8

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