The ultimate goal of this research is to understand the cellular mechanisms underlying electrolyte and fluid transport in salivary glands. A related goal is to understand how these transport processes are affected in cystic fibrosis.
The specific aims are: 1. To use the electron microprobe technique to measure elemental concentrations of Na, Mg, P, S, Cl, K and Ca in basal and apical cytoplasm, secretory granules, nuclei, mitochondria, lateral intercellular spaces and lumen of nonstimulated rat parotid gland acini. 2. To use the electron microprobe technique to measure the above elemental concentrations in the listed intracellular and extracellular spaces following induction of salivation with cholinergic and Alpha and Beta-adrenergic secretagogues. 3. To use the measured Na, Cl and other elemental concentrations listed above to test for the transcellular and paracellular electrolyte changes, and accompanying water fluxes, that are predicted by the solute-solvent coupled hypothesis for water flow in epithelial tissues. 4. To perform similar elemental analyses in intercalated and striated ducts from parotid glands in order to determine if there are appreciable electrolyte or water fluxes across these structures. 5. To perform similar analyses in parotid glands from reserpinized animals to determine how these electrolyte and water transport mechanisms are affected. These findings will contribute to our understanding of salivary gland physiology and of transport changes in the reserpinized rat--a model for cystic fibrosis in man.

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Research Project (R01)
Project #
5R01DE006373-03
Application #
3219959
Study Section
Oral Biology and Medicine Study Section (OBM)
Project Start
1983-03-01
Project End
1986-08-31
Budget Start
1985-03-01
Budget End
1986-08-31
Support Year
3
Fiscal Year
1985
Total Cost
Indirect Cost
Name
University of Washington
Department
Type
Schools of Dentistry/Oral Hygn
DUNS #
135646524
City
Seattle
State
WA
Country
United States
Zip Code
98195
Izutsu, K T; Cantino, M E; Johnson, D E (1994) A review of electron probe X-ray microanalysis studies of salivary gland cells. Microsc Res Tech 27:71-9
Wong, J G; Izutsu, K T; Robinovitch, M R et al. (1991) Microprobe analysis of maturation-related elemental changes in rat parotid secretory granules. Am J Physiol 261:C1033-41
Izutsu, K; Wilkinson, L; Oda, D et al. (1991) Comparison of elemental concentrations in the acinar cells of the human labial salivary gland. Arch Oral Biol 36:727-35
Allan, B J; Izutsu, K T; Ramsey, B W et al. (1990) Cyclic nucleotide responses in control and cystic fibrosis labial glands. Am J Physiol 258:R1320-6
Izutsu, K T; Ensign, W Y; Ramsey, B W et al. (1989) Potassium release in labial glands from controls and patients with cystic fibrosis. Lab Invest 60:158-60
Goddard, M K; Izutsu, K T; Johnson, D E et al. (1988) Evidence for two conductance/exchange pathways for chloride in rat parotid secretory granules. Biochem Biophys Res Commun 155:984-9
Johnson, D; Izutsu, K; Cantino, M et al. (1988) High spatial resolution spectroscopy in the elemental microanalysis and imaging of biological systems. Ultramicroscopy 24:221-35
Izutsu, K T; Schubert, M M; Truelove, E L et al. (1987) Use of human minor salivary glands in basic and applied secretion research. J Dent Res 66 Spec No:654-9
Izutsu, K T; Johnson, D E; Goddard, M (1987) Intracellular elemental concentrations in resting and secreting rat parotid glands. J Dent Res 66:537-40
Izutsu, K T; Johnson, D E (1986) Changes in elemental concentrations of rat parotid acinar cells following pilocarpine stimulation. J Physiol 381:297-309

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