Streptococcus mutans is recognized as the etiologic agent responsible for dental caries, and research on this organism has been promoted in order to better understand ways in which caries can be decreased. We have constructed two different gene libraries of the S. mutans chromose, and the availability of these genes allow a comprehensive study of the biological, chemical, and genetic properties of S. mutans surface protein antigens. The overall goal of this research is to define the surface protein antigens of S. mutans and elucidate their role in adherence or aggregation and contribution to dental caries.
The specific aims of this study are to: 1) Identify and characterize the specific protein surface antigens of S. mutans. 2) Assess the role of each of the proteins individually and in groups in adherence and/or aggregation. 3) Determine which protein, if any, is responsible for the observed cross reactivity with human heart tissue; and 4) Study the genetic organization and regulation of these surface proteins. The results from this research should facilitate the study of individual S. mutans surface protein antigens and identify those proteins involved in adherence and aggregation. Some of these proteins could serve as candidate antigens for a dental caries vaccine. Additionally, identification of any possible proteins responsible for human heart cross reactivity is an important consideration for the development of a safe caries vaccine. Finally, a genetic approach to the organization and regulation of S. mutans surface proteins can only enhance our understanding of the overall biology of these organisms.

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Research Project (R01)
Project #
5R01DE007836-02
Application #
3221618
Study Section
Oral Biology and Medicine Study Section (OBM)
Project Start
1986-02-01
Project End
1988-08-31
Budget Start
1987-01-01
Budget End
1988-08-31
Support Year
2
Fiscal Year
1987
Total Cost
Indirect Cost
Name
University of South Florida
Department
Type
Schools of Medicine
DUNS #
City
Tampa
State
FL
Country
United States
Zip Code
33612
Han, Thomas K; Zhu, Zhiwen; Dao, My Lien (2004) Identification, molecular cloning, and sequence analysis of a deoxyribose aldolase in Streptococcus mutans GS-5. Curr Microbiol 48:230-6
Han, T K; Yoder, S; Cao, C et al. (2001) Expression of Streptococcus mutans wall-associated protein A gene in Chinese hamster ovary cells: prospect for a dental caries DNA vaccine. DNA Cell Biol 20:595-601
Jackson, R J; Lim, D V; Dao, M L (1997) Identification and analysis of a collagenolytic activity in Streptococcus mutans. Curr Microbiol 34:49-54
Puerta, L; Caraballo, L; Fernandez-Caldas, E et al. (1996) Nucleotide sequence analysis of a complementary DNA coding for a Blomia tropicalis allergen. J Allergy Clin Immunol 98:932-7
Jackson, R J; Dao, M L; Lim, D V (1994) Cell-associated collagenolytic activity by group B streptococci. Infect Immun 62:5647-51
Qian, H; Dao, M L (1993) Inactivation of the Streptococcus mutans wall-associated protein A gene (wapA) results in a decrease in sucrose-dependent adherence and aggregation. Infect Immun 61:5021-8
Dao, M L; Chavez, C; Hirachi, Y et al. (1989) Molecular cloning of the Streptococcus mutans gene specifying antigen A. Infect Immun 57:3372-6
Dao, M L; Shao, R; Risley, J et al. (1989) Influence of chronic energy intake restriction on intestinal alkaline phosphatase in C3H/Bi mice and autoimmune-prone MRL/lpr,lpr mice. J Nutr 119:2017-22
Ferretti, J J; Russell, R R; Dao, M L (1989) Sequence analysis of the wall-associated protein precursor of Streptococcus mutans antigen A. Mol Microbiol 3:469-78