Proteoglycans are normal components of connective tissues and are believed to play an important role in the regulation and maintenance of the structural integrity of periodontal tissues. During periodontal disease a gradual dissolution of the connective tissue macromolecules takes place which may influence the concentration and composition of proteoglycans in these tissues. In order to understand the role of proteoglycans in healthy and diseased oral tissues the following important questions need to be answered: (1) which are the specific proteoglycans of oral tissues? (2) are these specific proteoglycans characteristic of various oral tissues? (3) How are these proteoglycans synthesized? We propose to seek answers to these questions through studies on structure and biosynthesis of proteoglycans of human gingival fibroblasts and of an established rat oral mucosal keratinocyte cell line. The cells will be incubated with radioactive sulfate, glucosamine or leucine and the labeled proteoglycans will be isolated and characterized. Monoclonal and polyclonal antibodies will be prepared against dermatan sulfate proteoglycans of soft connective tissues and heparan sulfate proteoglycans synthesized by keratinocytes. These antibodies will be utilized in the study of the biosynthesis of dermatan sulfate proteoglycans of human gingival fibroblasts and heparan sulfate proteoglycans synthesized by rat keratinocytes in culture. Pulse-chase label experiments in conjunction with immunoprecipitation will be carried out to determine the kinetics of the synthesis and processing of the proteoglycans and secretion of the completed proteoglycans into the extracellular matrix.

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Research Project (R01)
Project #
5R01DE008466-02
Application #
3222181
Study Section
Oral Biology and Medicine Study Section (OBM)
Project Start
1988-05-01
Project End
1991-04-30
Budget Start
1989-05-01
Budget End
1990-04-30
Support Year
2
Fiscal Year
1989
Total Cost
Indirect Cost
Name
University of Alabama Birmingham
Department
Type
Schools of Dentistry/Oral Hygn
DUNS #
004514360
City
Birmingham
State
AL
Country
United States
Zip Code
35294
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Mao, J J; Rahemtulla, F; Scott, P G (1998) Proteoglycan expression in the rat temporomandibular joint in response to unilateral bite raise. J Dent Res 77:1520-8
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Bignami, A; Perides, G; Rahemtulla, F (1993) Versican, a hyaluronate-binding proteoglycan of embryonal precartilaginous mesenchyma, is mainly expressed postnatally in rat brain. J Neurosci Res 34:97-106
Tona, A; Perides, G; Rahemtulla, F et al. (1993) Extracellular matrix in regenerating rat sciatic nerve: a comparative study on the localization of laminin, hyaluronic acid, and chondroitin sulfate proteoglycans, including versican. J Histochem Cytochem 41:593-9
Perides, G; Erickson, H P; Rahemtulla, F et al. (1993) Colocalization of tenascin with versican, a hyaluronate-binding chondroitin sulfate proteoglycan. Anat Embryol (Berl) 188:467-79
Perides, G; Rahemtulla, F; Lane, W S et al. (1992) Isolation of a large aggregating proteoglycan from human brain. J Biol Chem 267:23883-7
Larjava, H; Hakkinen, L; Rahemtulla, F (1992) A biochemical analysis of human periodontal tissue proteoglycans. Biochem J 284 ( Pt 1):267-74
Bignami, A; Asher, R; Perides, G et al. (1992) The extracellular matrix of cerebral gray matter: Golgi's pericellular net and Nissl's nervosen grau revisited. Int J Dev Neurosci 10:291-9

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