The broad objective of this proposal is to assess microbial risk factors for the initial periodontal lesion.
The specific aims will determine whether putative microbial risk markers for periodontal lesions could also be risk factors for initial lesions. Also, the association between the role of an altered interleukin-1 (IL-1) genotype, with increased expression of IL-1a and IL-1b in gingival crevicular fluid, and prevalence and progression of periodontitis will be determined. Reliable risk factors for initial lesions can then be used to identify susceptible individuals either before or in early phases of disease. Preventive intervention before tissue destruction occurs would be better, and less expensive, than treating diseased sites. Furthermore, if periodontal pathogens and mechanisms of pathogenesis of initial lesions could be defined, other research and treatment studies can be targeted to the appropriate species and mediators of tissue destruction. Putative pathogens and cytokines in 300 subjects will be examined using new molecular biology technologies for rapid assays of species, including new spirochete taxa, detection of cytokines and altered genotype for cytokine production.
Aim 1 examines the prevalence of subjects with microbial risk markers for periodontal loss or an altered IL-1 genotype, in a cross-sectional study comparing subjects with minimal periodontal attachment loss (cases) with periodontally healthy subjects (controls). The hypothesis to be tested is that the risk (or suspected) marker for periodontal attachment loss will be higher in cases than in healthy controls.
Aim 2 is a prospective cohort study monitoring 200 subjects selected from the first aim, to determine whether subjects with microbial risk markers for attachment loss or an altered IL-1 genotype demonstrate more extensive attachment loss than subjects without these risk markers. Subjects will undergo comprehensive clinical and microbial monitoring with measurements repeated at 6-month intervals for two years. Clinical characteristics of initial lesion subjects, including different patterns of attachment loss will be defined.
The third aim will evaluate site-specific associations of initial periodontal lesions and the cultivable subgingival microbiota, cultivated and uncultivated taxa of spirochetes, and cytokines IL-1a and IL-1b. Microbiologic, genetic and clinical information from this proposal will significantly advance understanding of the microbial etiology of initial periodontitis, which will have profound significance for basic studies in periodontal research.
|Stashenko, Philip; Van Dyke, Thomas; Tully, Patrice et al. (2011) Inflammation and genetic risk indicators for early periodontitis in adults. J Periodontol 82:588-96|
|Tanner, Anne C R; Kent Jr, Ralph; Kanasi, Eleni et al. (2007) Clinical characteristics and microbiota of progressing slight chronic periodontitis in adults. J Clin Periodontol 34:917-30|
|Tanner, A C R; Paster, B J; Lu, S C et al. (2006) Subgingival and tongue microbiota during early periodontitis. J Dent Res 85:318-23|
|Tanner, Anne C R; Kent Jr, Ralph; Van Dyke, Thomas et al. (2005) Clinical and other risk indicators for early periodontitis in adults. J Periodontol 76:573-81|
|Downes, Julia; Sutcliffe, Iain; Tanner, Anne C R et al. (2005) Prevotella marshii sp. nov. and Prevotella baroniae sp. nov., isolated from the human oral cavity. Int J Syst Evol Microbiol 55:1551-5|
|Hughes, C V; Malki, G; Loo, C Y et al. (2003) Cloning and expression of alpha-D-glucosidase and N-acetyl-beta-glucosaminidase from the periodontal pathogen, Tannerella forsythensis (Bacteroides forsythus). Oral Microbiol Immunol 18:309-12|
|Paster, Bruce J; Russell, Meaghan K; Alpagot, Tamer et al. (2002) Bacterial diversity in necrotizing ulcerative periodontitis in HIV-positive subjects. Ann Periodontol 7:8-16|
|Yang, E Y; Tanner, A C R; Milgrom, P et al. (2002) Periodontal pathogen detection in gingiva/tooth and tongue flora samples from 18- to 48-month-old children and periodontal status of their mothers. Oral Microbiol Immunol 17:55-9|
|Tanner, A C R; Milgrom, P M; Kent Jr, R et al. (2002) The microbiota of young children from tooth and tongue samples. J Dent Res 81:53-7|
|Tanner, A C R; Milgrom, P M; Kent Jr, R et al. (2002) Similarity of the oral microbiota of pre-school children with that of their caregivers in a population-based study. Oral Microbiol Immunol 17:379-87|
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