The long term objectives of this proposal are to define the microbial complexes that are mos compatible with periodontal health and devise therapeutic and/or preventive strategies to produce the desired plaque composition.
Specific Aim 1 will determine the composition of supra- and subgingival plaque, the associations among species both within and between supra- and subgingival plaques, and seek differences between plaques from healthy and diseased individuals. In this cross-sectional study, 60 periodontitis and 30 periodontally healthy subjects will be assessed clinically at 6 periodontal sites per tooth. Supragingival plaque and subgingival plaque samples will be taken separately from the mesial aspect of each tooth and evaluated individually for their content of 80 bacterial species using checkerboard DNA-DNA hybridization providing 403,200 bacterial counts. Supragingival plaque control is thought to aid in the contro of periodontal infections, possibly by affecting subgingival plaque composition.
Specific Aim 2 will determine the effect of repeated professional supragingival plaque removal on clinical parameters and subgingival plaque composition. In this longitudinal study, the 60 periodontitis patients from Specific Aim 1 will receive scaling and root planning (SRP) performed under local anesthesia. Thirty of these subjects, selected randomly, will receive professional supragingival plaque removal at weekly intervals for a period of months. All subjects will be evaluated clinically and for subgingival plaque composition (as described for Specific Aim 1) at 3, 6, and 12 months post SRP. Thus, an additional 403,200 bacterial counts will be determined. Data will be used to evaluate the effect of supragingival plaque control on the composition of subgingival plaque and the nature of the plaque composition associated with periodontal stability. The proposed studies will clarify the composition of supragingival plaque, extend the knowledge of subgingival plaque and provide a database for examining important bacterial associations in plaque. In addition the importance and biological basis of supragingival plaque control in the treatment and prevention of periodontal diseases and in maintaining stability of the periodontium will be determined.

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Research Project (R01)
Project #
1R01DE012108-01A2
Application #
2697638
Study Section
Oral Biology and Medicine Subcommittee 1 (OBM)
Project Start
1998-09-01
Project End
2001-06-30
Budget Start
1998-09-01
Budget End
1999-06-30
Support Year
1
Fiscal Year
1998
Total Cost
Indirect Cost
Name
Forsyth Institute
Department
Type
DUNS #
City
Boston
State
MA
Country
United States
Zip Code
02142
Tavares, W L F; Neves de Brito, L C; Teles, R P et al. (2011) Microbiota of deciduous endodontic infections analysed by MDA and Checkerboard DNA-DNA hybridization. Int Endod J 44:225-35
Teles, F R F; Teles, R P; Siegelin, Y et al. (2011) RNA-oligonucleotide quantification technique (ROQT) for the enumeration of uncultivated bacterial species in subgingival biofilms. Mol Oral Microbiol 26:127-39
Haffajee, A D; Teles, R P; Patel, M R et al. (2009) Factors affecting human supragingival biofilm composition. II. Tooth position. J Periodontal Res 44:520-8
Haffajee, A D; Teles, R P; Patel, M R et al. (2009) Factors affecting human supragingival biofilm composition. I. Plaque mass. J Periodontal Res 44:511-9
Bogren, Anna; Teles, Ricardo; Torresyap, Gay et al. (2007) A three-year prospective study of adult subjects with gingivitis. I: clinical periodontal parameters. J Clin Periodontol 34:1-6
Brito, L C N; Teles, F R; Teles, R P et al. (2007) Use of multiple-displacement amplification and checkerboard DNA-DNA hybridization to examine the microbiota of endodontic infections. J Clin Microbiol 45:3039-49
Teles, F; Haffajee, A D; Socransky, S S (2007) Multiple displacement amplification as an aid in checkerboard DNA-DNA hybridization. Oral Microbiol Immunol 22:118-25
Teles, R P; Bogren, A; Patel, M et al. (2007) A three-year prospective study of adult subjects with gingivitis II: microbiological parameters. J Clin Periodontol 34:7-17
Haffajee, Anne D; Teles, Ricardo P; Socransky, Sigmund S (2006) The effect of periodontal therapy on the composition of the subgingival microbiota. Periodontol 2000 42:219-58
Haffajee, A D; Teles, R P; Socransky, S S (2006) Association of Eubacterium nodatum and Treponema denticola with human periodontitis lesions. Oral Microbiol Immunol 21:269-82

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