The applicant proposes to determine the role of epithelial cell polarity in infection by EBV. Little is known about the early, molecular or otherwise, events in EBV infection, when a transient epithelial phase of infection is presumed to occur or what favors the appearance of epithelial diseases such as OHL, NPL or Burkitt's lymphoma associated with EBV. In preliminary results the applicant provides data to suggest that epithelial cell polarity may play a role, whether the uptake of EBV is mediated by the EBV receptor CR2 or by IgA modulation of infection. To address this issue the applicant has developed a MDCK cell line transformed with either (i) CDNA encoding the rabbit polymeric immunoglobulin receptor (pIgR) or (ii) CD21 (CR2) CDNA expression vector (PR4 CR2). These matched MDCK cell transformants will allow the investigators to follow the fate of EBV strains Bas.8 P3HR-1K and Akata following entry of epithelial cells by each pathway ie CR2 mediated or through IgA modulation. The investigators can examine the transcriptional program and fate of virion DNA as well as cell morphology and physiology via viral infection through both routes of entry. The investigators plan to determine whether EBV induces interleukin-10 (Il-10) in epithelial cells by ELISA, biological essays and eventually RT-PCR in collaboration.

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Research Project (R01)
Project #
5R01DE012187-05
Application #
6176107
Study Section
Special Emphasis Panel (ZDE1-YS (31))
Program Officer
Mangan, Dennis F
Project Start
1996-09-30
Project End
2002-07-31
Budget Start
2000-08-01
Budget End
2002-07-31
Support Year
5
Fiscal Year
2000
Total Cost
$219,839
Indirect Cost
Name
Louisiana State University Hsc Shreveport
Department
Microbiology/Immun/Virology
Type
Schools of Medicine
DUNS #
095439774
City
Shreveport
State
LA
Country
United States
Zip Code
71103
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Gan, Yan-Jun; Razzouk, Bassem I; Su, Tao et al. (2002) A defective, rearranged Epstein-Barr virus genome in EBER-negative and EBER-positive Hodgkin's disease. Am J Pathol 160:781-6
Chodosh, J; Gan, Y j; Holder, V P et al. (2000) Patterned entry and egress by Epstein-Barr virus in polarized CR2-positive epithelial cells. Virology 266:387-96
Slobod, K S; Sandlund, J T; Spiegel, P H et al. (2000) Molecular evidence of ocular Epstein-Barr virus infection. Clin Infect Dis 31:184-8