The purpose of this study is to delineate the molecular anatomy of mitochondrial genetic changes in head and neck cancer development. Mitochondrial mutations and genetic alterations have been found in head and neck cancer and mitochondrial dysfunction, DNA damage, and apoptotic dysregulation are found in multiple solid tumors. The entire 16 KB mitochondrial genome will be tested using conventional PCR based and novel array based sequencing assays in primary head and neck cancers and premalignant lesions. These alterations will be placed in the context of a genetic progression model for head and neck cancer to determine mitochondrial alterations that occur early in progression. Identified or engineered mutations in cell lines will be tested to understand their impact on mitochondrial biology and their role in cancer progression. Ultimately, identified mitochondrial alterations will be used as markers for molecular detection approaches using a novel, fluorescence based gap ligase chain reaction assay. ? ?

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Research Project (R01)
Project #
5R01DE015939-03
Application #
7010344
Study Section
Special Emphasis Panel (ZDE1-PZ (15))
Program Officer
Shirazi, Yasaman
Project Start
2004-04-01
Project End
2008-02-28
Budget Start
2006-03-01
Budget End
2008-02-28
Support Year
3
Fiscal Year
2006
Total Cost
$399,144
Indirect Cost
Name
Johns Hopkins University
Department
Otolaryngology
Type
Schools of Medicine
DUNS #
001910777
City
Baltimore
State
MD
Country
United States
Zip Code
21218
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