Survival rates for oral cancer patients have remained unchanged in the past several decades largely because of the late identification of the disease and the high rate of local recurrence after treatment. We have used the unique medical infrastructure in British Columbia to establish one of the largest cohort studies of dysplasia and cancer patients worldwide in order to systematically follow changes in clinical and pathological parameters, to evaluate established microsatellite markers for prognostic significance alone and in combination with these features, and to develop new genetic tools for risk prediction using genomic profiles generated from samples collected from the study. As yet, little research has been done on alternate approaches that would use different technology in conjunction with these molecular approaches to create an integrated plan for patient assessment and management that would be rapid and cost-effective. This proposal will evaluate 3 innovative, potentially complementary visualization and imaging devices (the VELScope, the ClearCyte and the Getafics, alone and in combination, for early detection and follow-up of oral premalignant lesions (OPLs) and oral cancer. The VELScope is a direct visualization device which makes use of tissue autofluorescence to detect and delineate abnormal lesions. The ClearCyte and Getafics are quantitative imaging microscopy systems. The ClearCyte is a fully automated imaging cytometry system which can automatically scan an entire slide and collect images and data from all the cells present. Getafics is a semi-automated imaging microscopy workstation which collects images of sectioned material and analyzes individual cell nuclei and their spatial arrangement within the tissue imaged. The proposed study will use patients enrolled in the ongoing longitudinal study as well as a cohort accrued from a highrisk community clinic. The study will: 1) Determine the prevalence of alterations detected by the devices as well as sensitivity/specificity for high-grade OPLs and cancer (Study Aim 1); 2) Determine the relationship between characteristics observed with these devices at first assessment and throughout follow-up with the development of adverse outcomes (progression or recurrence) (Study Aim 3); and 3) Determine whether the integration of these devices with molecular tests and clinicopathological features will facilitate the identification of critical changes earlier and with greater specificity (Study Aim 3). This information will be used to guide the identification and risk assessment of community patients and their triage to regional and cancer hospitals in a Province-wide clinical network being established by the British Columbia Oral Cancer Prevention Program in BC.

Agency
National Institute of Health (NIH)
Institute
National Institute of Dental & Craniofacial Research (NIDCR)
Type
Research Project (R01)
Project #
1R01DE017013-01
Application #
6964198
Study Section
Medical Imaging Study Section (MEDI)
Program Officer
Shirazi, Yasaman
Project Start
2005-09-02
Project End
2010-08-31
Budget Start
2005-09-02
Budget End
2006-08-31
Support Year
1
Fiscal Year
2005
Total Cost
$368,922
Indirect Cost
Name
British Columbia Cancer Agency
Department
Type
DUNS #
209137736
City
Vancouver
State
BC
Country
Canada
Zip Code
V5 1-L3
Rock, L D; Rosin, M P; Zhang, L et al. (2018) Characterization of epithelial oral dysplasia in non-smokers: First steps towards precision medicine. Oral Oncol 78:119-125
Zhang, Lewei; Lubpairee, Tarinee; Laronde, Denise M et al. (2016) Should severe epithelial dysplasia be treated? Oral Oncol 60:125-9
Baik, Jonathan; Ye, Qian; Zhang, Lewei et al. (2014) Automated classification of oral premalignant lesions using image cytometry and Random Forests-based algorithms. Cell Oncol (Dordr) 37:193-202
Laronde, Denise M; Williams, P M; Hislop, T G et al. (2014) Decision making on detection and triage of oral mucosa lesions in community dental practices: screening decisions and referral. Community Dent Oral Epidemiol 42:375-84
Martinez, Victor D; MacAulay, Calum E; Guillaud, Martial et al. (2014) Targeting of chemoprevention to high-risk potentially malignant oral lesions: challenges and opportunities. Oral Oncol 50:1123-30
Laronde, Denise M; Williams, P M; Hislop, T G et al. (2014) Influence of fluorescence on screening decisions for oral mucosal lesions in community dental practices. J Oral Pathol Med 43:7-13
Rosin, Miriam P; Zhang, Lewei; Mao, Li (2013) Predicting progression of oral dysplasia--response. Cancer Prev Res (Phila) 6:616
Zhang, Lewei; Poh, Catherine F; Williams, Michele et al. (2012) Loss of heterozygosity (LOH) profiles--validated risk predictors for progression to oral cancer. Cancer Prev Res (Phila) 5:1081-9
Poh, C F; Zhu, Y; Chen, E et al. (2012) Unique FISH patterns associated with cancer progression of oral dysplasia. J Dent Res 91:52-7
Poh, Catherine F; MacAulay, Calum E; Laronde, Denise M et al. (2011) Squamous cell carcinoma and precursor lesions: diagnosis and screening in a technical era. Periodontol 2000 57:73-88

Showing the most recent 10 out of 25 publications