The overall objective of this proposed research is to evaluate the validity, reliability and repeatability of polarization sensitive optical coherence tomography (PS-OCT) as a non-invasive optical device for the accurate and early clinical assessment of tooth demineralization and remineralization. The central hypothesis underlying this proposal is that depth-resolved changes in the magnitude of light scattering of dental hard tissues at 1310-nm, that occur upon demineralization and remineralization, can be imaged and quantified in vivo using PS-OCT and that those changes can be correlated with the structural changes and relative mineral loss measured using polarized-light microscopy and microradiography. The overall objective of this proposal will be achieved through the following specific aims. (1) To assemble and test a hand-held scanner incorporating a high-resolution probe for the clinical imaging of surface and sub-surface demineralization using PS-OCT. (2): To test the hypothesis that PS-OCT can be used to measure and quantify the early demineralization and remineralization of enamel, dentin and cementum in vitro using simulated caries models and natural lesions on extracted teeth. (3): To validate PS-OCT for the accurate and early clinical assessment of tooth surface and sub-surface demineralization by in vivo measurements using the hand-held scanner assembled and tested in aim#1. These studies and future clinical studies including those involving the important occlusal and root surfaces and those involving both tooth demineralization and remineralization will validate PS-OCT as a clinical tool for the accurate and early clinical assessment of tooth demineralization and remineralization. This is expected to lead to the clinical implementation of PS-OCT for the assessment and monitoring of the activity and severity of early carious lesions without the use of ionizing radiation, leading to an increased efficiency of caries clinical trials and a marked reduction in their scope and cost. Moreover, it is highly probable that this proposed imaging technology would enable the clinician to detect and quantify the severity of occlusal lesions in enamel that are not resolvable with conventional radiography due to attenuation by the surrounding sound tissue structure. This latter benefit would be a major advance in preventive dentistry as it would signal the need for early chemical therapeutic intervention and be able to monitor the subsequent success of that intervention. ? ? ?

National Institute of Health (NIH)
National Institute of Dental & Craniofacial Research (NIDCR)
Research Project (R01)
Project #
Application #
Study Section
Special Emphasis Panel (ZDE1-YL (60))
Program Officer
Drummond, James A
Project Start
Project End
Budget Start
Budget End
Support Year
Fiscal Year
Total Cost
Indirect Cost
University of California San Francisco
Schools of Dentistry
San Francisco
United States
Zip Code
Kim, Jin Wan; Lee, Raymond; Chan, Kenneth H et al. (2017) Influence of a pulsed CO2 laser operating at 9.4???m on the surface morphology, reflectivity, and acid resistance of dental enamel below the threshold for melting. J Biomed Opt 22:28001
Lee, Raymond; Chan, Kenneth H; Jew, Jamison et al. (2017) Synergistic effect of fluoride and laser irradiation for the inhibition of the demineralization of dental enamel. Proc SPIE Int Soc Opt Eng 10044:
Simon, Jacob C; Kang, Hobin; Staninec, Michal et al. (2017) Near-IR and CP-OCT imaging of suspected occlusal caries lesions. Lasers Surg Med 49:215-224
Lee, Robert C; Darling, Cynthia L; Staninec, Michal et al. (2017) Activity assessment of root caries lesions with thermal and near-IR imaging methods. J Biophotonics 10:433-445
Kang, Hobin; Darling, Cynthia L; Fried, Daniel (2016) Enhancement of OCT images with vinyl polysiloxane (VPS). Proc SPIE Int Soc Opt Eng 9692:
Chan, Kenneth H; Tom, Henry; Lee, Robert C et al. (2016) Clinical monitoring of smooth surface enamel lesions using CP-OCT during nonsurgical intervention. Lasers Surg Med 48:915-923
Lee, Robert C; Staninec, Michal; Le, Oanh et al. (2016) Infrared Methods for Assessment of the Activity of Natural Enamel Caries Lesions. IEEE J Sel Top Quantum Electron 22:
Kang, Hobin; Darling, Cynthia L; Fried, Daniel (2016) Use of an optical clearing agent to enhance the visibility of subsurface structures and lesions from tooth occlusal surfaces. J Biomed Opt 21:081206
Tom, Henry; Chan, Kenneth H; Darling, Cynthia L et al. (2016) Near-IR image-guided laser ablation of demineralization on tooth occlusal surfaces. Lasers Surg Med 48:52-61
Tam, Wilson; Lee, Robert C; Lin, Brent et al. (2016) Assessment of simulated lesions on primary teeth with near-infrared imaging. Proc SPIE Int Soc Opt Eng 9692:

Showing the most recent 10 out of 54 publications