A series of spectoscopic, chemical and crystallographic studies will be conducted on three highly purified vitamin B6-dependent enzymes: cytosolic aspartate aminotransferase of pig hearts (AspAT), tryptophanase of Escherichia coli and glutamate decarboxylase of E. coli. The goals will be to provide understanding of basic mechanisms of pyridoxal phosphate- dependent catalysis and to assist in the design of useful enzyme-activated inhibitors. (1) We will conduct a series of microspectrophotometric measurements on crystals of aspartate aminotransferase. We will measure binding constants within crystals for inhibitors and natural substrates. (2) We will collaborate in determining structures of substrate and inhibitor complexes of this enzyme. We will make special efforts to stabilize the erythro-3-hydroxyaspartate complex and to deduce its structure. (3) We will separate individual species of the self-inactivated beta subform of AspAT by isoelectric focusing and will crystallize at least the major species and some other less abundant forms. This homogeneous beta form will be analyzed by X-ray diffraction and will be used by us in microspectrophotometry for a variety of experiments. We will characterize an addition major compound released from the inactivated subunit of the beta subform by heating. (4) The 6-fluoro derivatives of pyridoxal and pyridoxamine phosphates, incorporated in to the active sites of AspAT, tryptophanase, and bacterial glutamate decarboxylase, will be studied by 19F NMR spectroscopy. (5) We will try to crystallize AspAT inhibited by enzyme-activated inhibitors including beta-methyleneaspartate, serine sulfate, and acetylenic GABA. This should allow us to see directly the structures of different types of inhibited enzyme. The resulting knowledge will be important for rational design of drugs for inhibition of other transaminases. We also propose to do modeling of inhibitor action using an Evans & Sutherland graphics terminal. We will try series of spectroscopic studies on tryptophanase and glutamate decarboxylase aimed at elucidating the structures of intermediate species. We will also attempt to obtain crystals of these enzymes suitable for X-ray studies. (7) We will conduct nonenzymic studies on formation of Al3+ -stabilized quinonoid species in methanol and we will compare rates of alpha hydrogen removal in model systems with pyridoxal phosphate, its N-methyl derivatives and with 6-fluoropyridoxal phosphate.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK001549-35
Application #
3224324
Study Section
Metallobiochemistry Study Section (BMT)
Project Start
1975-08-01
Project End
1994-03-30
Budget Start
1992-04-01
Budget End
1993-03-31
Support Year
35
Fiscal Year
1992
Total Cost
Indirect Cost
Name
Iowa State University
Department
Type
Schools of Arts and Sciences
DUNS #
City
Ames
State
IA
Country
United States
Zip Code
50011
Metzler, D E; Metzler, C M; Scott, R D et al. (1994) NMR studies of 1H resonances in the 10-18-ppm range for aspartate aminotransferase from Escherichia coli. J Biol Chem 269:28027-33
Firsov, L M; Neustroev, K N; Aleshin, A E et al. (1994) NMR spectroscopy of exchangeable protons of glucoamylase and of complexes with inhibitors in the 9-15-ppm range. Eur J Biochem 223:293-302
Metzler, D E; Metzler, C M; Mollova, E T et al. (1994) NMR studies of 1H resonances in the 10-18-ppm range for cytosolic aspartate aminotransferase. J Biol Chem 269:28017-26
Chu, W C; Metzler, D E (1994) Enzymatically active truncated cat brain glutamate decarboxylase: expression, purification, and absorption spectrum. Arch Biochem Biophys 313:287-95
Metzler, C M; Viswanath, R; Metzler, D E (1991) Equilibria and absorption spectra of tryptophanase. J Biol Chem 266:9374-81
Kintanar, A; Metzler, C M; Metzler, D E et al. (1991) NMR observation of exchangeable protons of pyridoxal phosphate and histidine residues in cytosolic aspartate aminotransferase. J Biol Chem 266:17222-9
Metzler, C M; Metzler, D E; Kintanar, A et al. (1991) NMR spectra of exchangeable protons of pyridoxal phosphate-dependent enzymes. Biochem Biophys Res Commun 178:385-92
Taylor, J E; Metzler, D E; Arnone, A (1990) Modeling inhibitors in the active site of aspartate aminotransferase. Ann N Y Acad Sci 585:58-67
Miura, R; Metzler, C M; Metzler, D E (1989) Reactions of phosphonate analogs of pyridoxal phosphate with apo-aspartate aminotransferase. Arch Biochem Biophys 270:526-40
Metzler, C M; Mitra, J; Metzler, D E et al. (1988) Correlation of polarized absorption spectroscopic and X-ray diffraction studies of crystalline cytosolic aspartate aminotransferase of pig hearts. J Mol Biol 203:197-220

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