Abstract

The objectives of the proposed research constitutes a continuation of investigations on the transport of calcium and other mineral ions across the epithelium of the intestine. Emphasis will be given to different aspects of the problems of vitamin D action.
Specific aims are to: (a) determine the amino acid sequence of chick CaBP and its interactions, (b) investigate in detail alterations in phopholipid metabolism as related to 1,25(OH)2D3 action, (c) to study possible 1,25(OH)2D3-mediated changes in membrane fluidity and mobility of membrane components, (d) initiate studies on the use of epithelial cell lines to investigate aspects of 1,25(OH)2D3 action, (e) investigate the properties and characteristics of the ATP-dependent basal-lateral pump, (f) identify and investigate the properties and function of specific 1,25(OH)2D3-modulated proteins and lipids, (g) examine hormonal effects in a model in which chicks are given 1,25(OH)2D3 as their only source of vitamin D3, and (h) to determine possible alterations in calmodulin behavior in calcium, phosphorus and vitamin D3-deficiency.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK004652-27
Application #
3224448
Study Section
General Medicine B Study Section (GMB)
Project Start
1978-12-01
Project End
1988-11-30
Budget Start
1986-12-01
Budget End
1987-11-30
Support Year
27
Fiscal Year
1987
Total Cost
Indirect Cost

  Institution

Name
Cornell University
Department
Type
Schools of Veterinary Medicine
DUNS #
City
Ithaca
State
NY
Country
United States
Zip Code
14850

  Publications

Tolosa de Talamoni, N; Perez, A; Riis, R et al. (2002) Comparative immunolocalization of the plasma membrane calcium pump and calbindin D28K in chicken retina during embryonic development. Eur J Histochem 46:333-40
Fullmer, C S; Chandra, S; Smith, C A et al. (1996) Ion microscopic imaging of calcium during 1,25-dihydroxyvitamin D-mediated intestinal absorption. Histochem Cell Biol 106:215-22
Tolosa de Talamoni, N G (1996) Calcium and phosphorous deficiencies alter the lipid composition and fluidity of intestinal basolateral membranes. Comp Biochem Physiol A Physiol 115:309-15
Barr, S C; Han, W; Andrews, N W et al. (1996) A factor from Trypanosoma cruzi induces repetitive cytosolic free Ca2+ transients in isolated primary canine cardiac myocytes. Infect Immun 64:1770-7
McDonald, T J; Li, C; Wasserman, R H (1995) Distribution of calbindin-D28K in the brain of the fetal sheep in late gestation. Brain Res 675:303-15
Wasserman, R H; Fullmer, C S (1995) Vitamin D and intestinal calcium transport: facts, speculations and hypotheses. J Nutr 125:1971S-1979S
Pannabecker, T L; Chandler, J S; Wasserman, R H (1995) Vitamin-D-dependent transcriptional regulation of the intestinal plasma membrane calcium pump. Biochem Biophys Res Commun 213:499-505
Cai, Q; Tapper, D N; Gilmour Jr, R F et al. (1994) Modulation of the excitability of avian peripheral nerves by vitamin D: relation to calbindin-D28k, calcium status and lipid composition. Cell Calcium 15:401-10
Cai, Q; Chandler, J S; Wasserman, R H et al. (1993) Vitamin D and adaptation to dietary calcium and phosphate deficiencies increase intestinal plasma membrane calcium pump gene expression. Proc Natl Acad Sci U S A 90:1345-9
Corradino, R A (1993) Calbindin D28K regulation in precociously matured chick egg shell gland in vitro. Gen Comp Endocrinol 91:158-66

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