This proposed work is designed to elucidate the biochemical and/or physiological mechanisms in the brain mediating the regulation of food intake of animals fed disproportionate amounts of dietary amino acids. Our objective is to determine the location and the nature of the critical neural and/or other areas known to affect feeding behavior and the mediator, metabolic or otherwise, as well as the mode through which the control mechanism is triggered in response to dietary or experimental changes involving protein, amino acid, or other nutrients in disproportionate amounts. To achieve this goal, we intend to identify or establish the effects of injection or infusion either peripherally or centrally (through acute or chronic implant cannulas) of certain amino acids, neuropeptides, or certain drugs on the changes in feeding responses of normal rats or rats with lesions in critical neural areas, fed amino acid imbalanced or devoid diets or diets containing excessive quantities of protein or amino acids. Also, the changes in detailed feeding patterns will be determined with a computerized feeding monitoring system, and closely correlated with changes in biochemical and/or physiological parameters in plasma, brain or certain areas of the brain, cerebral spinal fluid or other tissues under conditions in which the food intake regulation mechanism is activated by dietary or other treatments or exogenously introduced substances as mentioned. Also, ablations of the extrahypothalamic areas (such as the area postrema and others) will be carried out to characterize the involvement of these areas in food intake regulation of animals fed disproportionate amounts of dietary amino acids. Thus far no system in the brain has been found that is responsive to excess dietary protein or amino acids. This should provide a better understanding of the mechanism mediating the food intake regulation of animals and men under conditions of disproportionality of dietary protein and amino acids.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK013252-18
Application #
3225012
Study Section
Nutrition Study Section (NTN)
Project Start
1976-05-01
Project End
1990-03-31
Budget Start
1988-04-01
Budget End
1990-03-31
Support Year
18
Fiscal Year
1988
Total Cost
Indirect Cost
Name
University of California Davis
Department
Type
Schools of Veterinary Medicine
DUNS #
094878337
City
Davis
State
CA
Country
United States
Zip Code
95618
Gietzen, D W; Beverly 3rd, J L (1992) Clonidine in the prepyriform cortex blocked anorectic response to amino acid imbalance. Am J Physiol 263:R885-90
Beverly, J L; Hrupka, B J; Gietzen, D W et al. (1991) Distribution of dietary limiting amino acid injected into the prepyriform cortex. Am J Physiol 260:R525-32
Beverly, J L; Gietzen, D W; Rogers, Q R (1990) Effect of dietary limiting amino acid in prepyriform cortex on food intake. Am J Physiol 259:R709-15
Beverly, J L; Gietzen, D W; Rogers, Q R (1990) Effect of dietary limiting amino acid in prepyriform cortex on meal patterns. Am J Physiol 259:R716-23
Semon, B A; Leung, P M; Rogers, Q R et al. (1989) Plasma and brain ammonia and amino acids in rats measured after feeding 75% casein or 28% egg white. J Nutr 119:1583-92
Semon, B A; Leung, P M; Rogers, Q R et al. (1989) Plasma ammonia, plasma, brain and liver amino acids and urea cycle enzyme activities in rats fed ammonium acetate. J Nutr 119:166-74
Semon, B A; Leung, P M; Rogers, Q R (1988) Effect of pre-feeding ammonium acetate on food intake of rats fed high protein diets. Physiol Behav 42:471-6
Semon, B A; Leung, P M; Rogers, Q R et al. (1988) Increase in plasma ammonia and amino acids when rats are fed a 44% casein diet. Physiol Behav 43:631-6
Semon, B A; Leung, P M; Rogers, Q R et al. (1987) Effect of type of protein on food intake of rats fed high protein diets. Physiol Behav 41:451-8