The goal of this research is to provide correlative morphological and biochemical information relating to pigment organelle movement in goldfish xanthophores and melanophores. The interactions of pigment organelles with the cytoskeleton during organelle translocation will be studied in two reversible and hormone-regulated systems, xanthophores and melanophores derived from the goldfish. The translocation of melanosomes in melanophores and of carotenoid droplets in xanthophores differ in several respects which make the two cells complementary models of intracellular motility. In order to explore the nature of organelle-cytoskeleton interactions in these cells and to determine how these interactions may be altered during organelle translocation, we propose to: (i) further characterize the organelles and the cytoskeletons biochemically; (ii) describe in more detail the morphological features of organelle translocation (both ultrastructurally and in living cells) and determine, if possible, which cytoskeletal components are physically associated with the migrating pigment organelles; (iii) establish in vitro systems which support the interactions between the pigment organelles and cytoskeleton (and components thereof) and with which these interactions can be characterized; (iv) confirm the results of in vitro analyses by raising and microinjecting monoclonal antibodies against relevant organelle and cytoskeletal proteins.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK013724-18
Application #
3225121
Study Section
Cellular Biology and Physiology Subcommittee 1 (CBY)
Project Start
1974-11-01
Project End
1989-11-30
Budget Start
1986-12-01
Budget End
1987-11-30
Support Year
18
Fiscal Year
1987
Total Cost
Indirect Cost
Name
Wayne State University
Department
Type
Schools of Arts and Sciences
DUNS #
City
Detroit
State
MI
Country
United States
Zip Code
48202
Peng, G; Taylor, J D; Tchen, T T (1994) Goldfish tyrosinase related protein I (TRP-1): deduced amino acid sequence from cDNA and comments on structural features. Pigment Cell Res 7:9-16
Taylor, J D; Kimler, V A; Tchen, T T (1991) A chromatophore index based upon the three-dimensional configuration of fish xanthophores and erythrophores. Pigment Cell Res 4:120-2
Shih, Y L; Chou, S C; Chi, C W et al. (1990) Tropic effect of dexamethasone on goldfish melanocytoma cells: induction of calcium-dependent but protein synthesis-independent morphological changes. Life Sci 47:313-8
Yu, F X; Taylor, J D; Tchen, T T (1990) Actin-dependent carotenoid droplet dispersion in permeabilized cultured goldfish xanthophores. Cell Motil Cytoskeleton 15:139-46
Wu, B Y; Yu, F X; Lynch, T J et al. (1990) Partial characterization of a carotenoid droplet ATPase and its possible significance in carotenoid droplet dispersion in goldfish xanthophores. Cell Motil Cytoskeleton 15:147-55
Chou, S C; Yang, C F; Kimler, V A et al. (1989) Reversible dedifferentiation and redifferentiation of a melanized cell line from a goldfish tumor. Cell Differ Dev 28:105-17
Chou, S C; Taylor, J D; Tchen, T T (1989) Epinephrine-induced pigment aggregation in goldfish melanophoroma cells: apparent involvement of an unknown second messenger. Pigment Cell Res 2:414-20
Chou, S C; Taylor, J D; Tchen, T T (1989) Isolation of melanized cell lines with stable phenotypes from a goldfish erythrophoroma cell line and cryopreservation of these cells by the use of autologous serum. In Vitro Cell Dev Biol 25:813-20
Yang, C F; Zeng, Z C; Chou, S C et al. (1989) Phosphorylation of the carotenoid droplet protein p57 by the catalytic subunit of cyclic adenosine monophosphate-dependent protein kinase and the effect of fluoride. Pigment Cell Res 2:408-13
Palazzo, R E; Lynch, T J; Lo, S J et al. (1989) Rearrangements of pterinosomes and cytoskeleton accompanying pigment dispersion in goldfish xanthophores. Cell Motil Cytoskeleton 13:9-20

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