We are attempting to extend our analysis of the regulation of glnA expression in K. aerogenes. We are isolating strains carrying the lac genes of E. coli fused to the glnA and glnG promoters in the wild type strain and in mutants with altered glnA expression due to mutations in the glnA region. The expression of these genes can then be studied in the presence and absence of their products. We are attempting to determine the manner of regulation of the asparagine synthetases coded by asnA and asnB. We are continuing our attempt to obtain mutants of B subtilis defective in catabolite repression.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK013894-17
Application #
3225140
Study Section
Biochemistry Study Section (BIO)
Project Start
1974-11-01
Project End
1990-03-31
Budget Start
1986-04-01
Budget End
1987-03-31
Support Year
17
Fiscal Year
1986
Total Cost
Indirect Cost
Name
Massachusetts Institute of Technology
Department
Type
Schools of Arts and Sciences
DUNS #
City
Cambridge
State
MA
Country
United States
Zip Code
02139
Magasanik, B (1993) The regulation of nitrogen utilization in enteric bacteria. J Cell Biochem 51:34-40
Ray, L; Claverie-Martin, F; Weglenski, P et al. (1990) Role of the promoter in activation of transcription by nitrogen regulator I phosphate in Escherichia coli. J Bacteriol 172:818-23
Weglenski, P; Ninfa, A J; Ueno-Nishio, S et al. (1989) Mutations in the glnG gene of Escherichia coli that result in increased activity of nitrogen regulator I. J Bacteriol 171:4479-85
Reitzer, L J; Bueno, R; Cheng, W D et al. (1987) Mutations that create new promoters suppress the sigma 54 dependence of glnA transcription in Escherichia coli. J Bacteriol 169:4279-84