Abstract

Since the association of rabbit muscle phosphofructokinase is apparently pH dependent, between pH 6.0 and 8.0, this variable will be carefully followed by sedimentation velocity, giving information on the difference between the affinities for H ion monomers and aggregates obtained by applying the linked function theory expressed by Wyman (Adv. Protein Chem. 19, 224, 1964). On the other hand, by keeping the solution at a constant pH and the activity of one of the solute species is varied then the equation becomes an expression for the differential of the ligand associated with monomer and aggregates. The ligands of interest are ATP, citrate and the fructose phosphates.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK021489-07
Application #
3226990
Study Section
Biophysics and Biophysical Chemistry B Study Section (BBCB)
Project Start
1979-05-01
Project End
1987-11-30
Budget Start
1985-12-01
Budget End
1986-11-30
Support Year
7
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
Saint Louis University
Department
Type
Schools of Medicine
DUNS #
City
Saint Louis
State
MO
Country
United States
Zip Code
63103

  Publications

Cheng, X; Friesen, R H; Lee, J C (1996) Effects of conserved residues on the regulation of rabbit muscle pyruvate kinase. J Biol Chem 271:6313-21
Cheng, X; Lee, J C (1994) Absolute requirement of cyclic nucleotide in the activation of the G141Q mutant cAMP receptor protein from Escherichia coli. J Biol Chem 269:30781-4
Chen, J; Surendran, R; Lee, J C et al. (1994) Construction of a dimeric repressor: dissection of subunit interfaces in Lac repressor. Biochemistry 33:1234-41
Cheng, X; Gonzalez, M L; Lee, J C (1993) Energetics of intersubunit and intrasubunit interactions of Escherichia coli adenosine cyclic 3',5'-phosphate receptor protein. Biochemistry 32:8130-9
Heyduk, T; Lee, J C (1992) Solution studies on the structure of bent DNA in the cAMP receptor protein-lac DNA complex. Biochemistry 31:5165-71
Heyduk, E; Heyduk, T; Lee, J C (1992) Global conformational changes in allosteric proteins. A study of Escherichia coli cAMP receptor protein and muscle pyruvate kinase. J Biol Chem 267:3200-4
Heyduk, E; Heyduk, T; Lee, J C (1992) Intersubunit communications in Escherichia coli cyclic AMP receptor protein: studies of the ligand binding domain. Biochemistry 31:3682-8
Consler, T G; Jennewein, M J; Cai, G Z et al. (1992) Energetics of allosteric regulation in muscle pyruvate kinase. Biochemistry 31:7870-8
Lin, T H; Quinn, T; Walsh, M et al. (1991) Avian myeloblastosis virus reverse transcriptase. Effect of glycerol on its hydrodynamic properties. J Biol Chem 266:1635-40
Cai, G Z; Lee, L L; Luther, M A et al. (1990) Regulation and quaternary structural changes in rabbit muscle phosphofructokinase. Biophys Chem 37:97-106

Showing the most recent 10 out of 17 publications