Abstract

The control of acid-base regulation by the kidney is critical in maintaining body electrolyte homeostasis. Many areas of the nephron participate in this control but little is known about the factors will affect collecting duct acidification. This nephron site is of critical importance since it is the location where the steepest pH gradients are set, where bicarbonate absorption is modulated and where important additions of ammonia and the titration of buffers occur. In this proposed research we will directly examine the acidification process along the inner medullary collecting duct and the various factors which affect acidification at this site. These studies are now possible because of the development of new microanalytic methods for the in vivi and in vitro measurement of pH, pCO2, total C02 and the other components of the acidification process and our ability to perform collections along the entire lenghth of the IMCD.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK028164-12
Application #
3228635
Study Section
General Medicine B Study Section (GMB)
Project Start
1981-09-01
Project End
1988-08-31
Budget Start
1986-09-01
Budget End
1988-08-31
Support Year
12
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
Boston University
Department
Type
Schools of Medicine
DUNS #
604483045
City
Boston
State
MA
Country
United States
Zip Code
02118

  Publications

Banerjee, A; Li, G; Alexander, E A et al. (2001) Role of SNAP-23 in trafficking of H+-ATPase in cultured inner medullary collecting duct cells. Am J Physiol Cell Physiol 280:C775-81
Alexander, E A; Brown, D; Shih, T et al. (1999) Effect of acidification on the location of H+-ATPase in cultured inner medullary collecting duct cells. Am J Physiol 276:C758-63
Banerjee, A; Shih, T; Alexander, E A et al. (1999) SNARE proteins regulate H(+)-ATPase redistribution to the apical membrane in rat renal inner medullary collecting duct cells. J Biol Chem 274:26518-22
Obrador, G; Yuan, H; Shih, T M et al. (1998) Characterization of anion exchangers in an inner medullary collecting duct cell line. J Am Soc Nephrol 9:746-54
Alexander, E A; Shih, T; Schwartz, J H (1997) H+ secretion is inhibited by clostridial toxins in an inner medullary collecting duct cell line. Am J Physiol 273:F1054-7
Schwartz, J H (1995) Renal acid-base transport: the regulatory role of the inner medullary collecting duct. Kidney Int 47:333-41
Schwartz, J H; Masino, S A; Nichols, R D et al. (1994) Intracellular modulation of acid secretion in rat inner medullary collecting duct cells. Am J Physiol 266:F94-101
Schwartz, G J; Brown, D; Mankus, R et al. (1994) Low pH enhances expression of carbonic anhydrase II by cultured rat inner medullary collecting duct cells. Am J Physiol 266:C508-14
Slotki, I; Schwartz, J H; Alexander, E A (1993) Interrelationship between cell pH and cell calcium in rat inner medullary collecting duct cells. Am J Physiol 265:C432-8
Mankus, R; Schwartz, J H; Alexander, E A (1993) Acidification adaptation in cultured inner medullary collecting duct cells. Am J Physiol 264:F765-9

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