Abstract

The specific aims of this proposal are to characterize two recently identified forms of pepsinogen I (PG I) that have been designated Big-Big PG I and Big PG I based on their higher molecular weights than the third form of PG I, now designated Little PG I, and to complete studies on serum pepsinogen II (PG II) levels. Big-Big PG I and Big PG I will be isolated and purified from human gastric mucosa by sequential gel exclusion chromatography, immunoadsorption, and ion exchange chromatography. Antibodies to these antigens will be raised in rabbits and, if necessary, made monospecific by immunoadsorption. The antibodies will be used to purify additional antigen and to develop specific radioimmunoassays for Big-Big PG I and Big PG I. The sequence of mucosal synthesis of the three forms of PG I, and their several electrophoretic constituents, will be determined in vitro by pulse-chase incubations of gastric mucosal biopsies followed by gel exclusion chromatography, polyacrylamide gel electrophoresis, and fluorography. The concentrations of Big-Big PG I, Big PG I, and PG II will be determined by radioimmunoassay in serum form control subjects and patients with disorders of the stomach and duodenum. The goals of this project are to determine the role of the pepsinogens in the pathogenesis of focal and diffuse disorders of the stomach and duodenum.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK032015-05
Application #
3230485
Study Section
General Medicine A Subcommittee 2 (GMA)
Project Start
1982-04-01
Project End
1988-11-30
Budget Start
1986-12-01
Budget End
1987-11-30
Support Year
5
Fiscal Year
1987
Total Cost
Indirect Cost

  Institution

Name
University of California Los Angeles
Department
Type
Schools of Medicine
DUNS #
119132785
City
Los Angeles
State
CA
Country
United States
Zip Code
90095

  Publications

Tenti, P; Aguzzi, A; Riva, C et al. (1992) Ovarian mucinous tumors frequently express markers of gastric, intestinal, and pancreatobiliary epithelial cells. Cancer 69:2131-42
Sessa, F; Bonato, M; Frigerio, B et al. (1990) Ductal cancers of the pancreas frequently express markers of gastrointestinal epithelial cells. Gastroenterology 98:1655-65
Fiocca, R; Villani, L; Tenti, P et al. (1990) The foveolar cell component of gastric cancer. Hum Pathol 21:260-70
Samloff, I M (1989) Peptic ulcer: the many proteinases of aggression. Gastroenterology 96:586-95
Lamers, C B; Rotter, J I; Jansen, J B et al. (1988) Serum pepsinogen I in familial multiple endocrine neoplasia type I. Dig Dis Sci 33:1274-6