There are an estimated 11 million diabetic patients in this country and approximately 80 percent are classified as Type II or non-insulin-dependent. Glucose intolerance is well recognized in both Type I and Type II diabetic patients, and has been attributed to both receptor and post-receptor defects. Since glucose intolerance is a major risk factor for the development of atherosclerosis our understanding of the mechanism involved and ways of reducing or reversing glucose intolerance is important for the health of this nation. The purpose of this proposal is to better understand the mechanisms involved in glucose transport in skeletal muscle and the roles played by both exercise and diet in regulating glucose transport. Previous studies have used nontarget tissues such as red blood cells or monocytes to evaluate changes in affinity and number of insulin receptors. Our initial data indicated that changes in these nontarget tissues do not refect changes in skeletal muscle insulin receptors following exercise training. Our studies are designed to test the effects of regular exercise and a low-fat diet on glucose tolerance, insulin binding to skeletal muscle sarcolemma (SL) membranes, and specific D-glucose transport by the sarcolemma vesicles. The total number and affinity of SL functional glucose transport units will be assessed by determining the D-glucose inhibitable class of cytochalasin B binding sites. We will also assess the ability of insulin to translocate the glucose transport units from the interior of the cell into the SL membrane. These studies will be done on normal rats as well as rats made diabetic by stroptozotocin treatment. The results should enhance our understanding of glucose transport in normal and diabetic muscle, separate differences between receptor and post-receptor defects and provide a scientific basis for the use of regular exercise and a low-fat diet by both Type I and Type II diabetic patients.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
1R01DK034326-01A1
Application #
3232657
Study Section
Respiratory and Applied Physiology Study Section (RAP)
Project Start
1986-01-01
Project End
1989-12-31
Budget Start
1986-01-01
Budget End
1986-12-31
Support Year
1
Fiscal Year
1986
Total Cost
Indirect Cost
Name
University of California Los Angeles
Department
Type
Schools of Arts and Sciences
DUNS #
119132785
City
Los Angeles
State
CA
Country
United States
Zip Code
90095
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Hou, J C; Salem, G J; Zernicke, R F et al. (1990) Structural and mechanical adaptations of immature trabecular bone to strenuous exercise. J Appl Physiol 69:1309-14
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Sternlicht, E; Barnard, R J; Grimditch, G K (1989) Beta-adrenergic receptors are not responsible for exercise stimulation of glucose transport. J Appl Physiol 66:2419-22
Sternlicht, E; Barnard, R J; Grimditch, G K (1988) Mechanism of insulin action on glucose transport in rat skeletal muscle. Am J Physiol 254:E633-8
Grimditch, G K; Barnard, R J; Hendricks, L et al. (1988) Peripheral insulin sensitivity as modified by diet and exercise training. Am J Clin Nutr 48:38-43

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