Abstract

The overall aim of this proposal is to advance the technique of mass spectrometry as it is related to steroid research and to offer an analytical service to researchers who lack the expertise or equipment necessary to carry out investigations of this type. Development of the technology will be in the field of Fast Atom Bombardment Mass Spectrometry. This technique allows the analysis of polar charged molecules so is ideally suited to the study of steroid sulfates and glucuronides. It is proposed to investigate ways of making this technique quantitative since only qualitative aspects have been described so far. Collaborative work with outside investigators requiring mass spectrometry will take principally two forms: (a) short studies on the analysis of synthetic steroids, isolated steroid metabolites, or total steroids in urine and plasma samples from individual patients of interest and (b) in depth studies in selected areas. These latter studies will be designed to use mass spectrometry and gas chromatography to elucidate some existing problems in endocrinology. Examples of current and on-going collaborative research include investigations into the synthesis and metabolism of 19-nor-DOC and a study of the anomalous steroid production of patients with juvenile hypertension.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK034400-08
Application #
3232740
Study Section
Special Emphasis Panel (SSS (M))
Project Start
1984-01-01
Project End
1991-12-31
Budget Start
1991-01-15
Budget End
1991-12-31
Support Year
8
Fiscal Year
1991
Total Cost
Indirect Cost

  Institution

Name
Children's Hospital & Res Ctr at Oakland
Department
Type
DUNS #
City
Oakland
State
CA
Country
United States
Zip Code
94609

  Publications

Witkowski, A; Witkowska, H E; Knudsen, J et al. (1999) Ether bond cleavage in an arylazido photoaffinity probe induced by ultraviolet light. Anal Biochem 267:412-5
Carr, A C; van den Berg, J J; Winterbourn, C C (1998) Differential reactivities of hypochlorous and hypobromous acids with purified Escherichia coli phospholipid: formation of haloamines and halohydrins. Biochim Biophys Acta 1392:254-64
Santner, S J; Albertson, B; Zhang, G Y et al. (1998) Comparative rates of androgen production and metabolism in Caucasian and Chinese subjects. J Clin Endocrinol Metab 83:2104-9
Rangan, V S; Serre, L; Witkowska, H E et al. (1997) Characterization of the malonyl-/acetyltransacylase domain of the multifunctional animal fatty acid synthase by expression in Escherichia coli and refolding in vitro. Protein Eng 10:561-6
Bearer, C; Emerson, R K; O'Riordan, M A et al. (1997) Maternal tobacco smoke exposure and persistent pulmonary hypertension of the newborn. Environ Health Perspect 105:202-6
Hellerstein, M K; Letscher, A; Schwarz, J M et al. (1997) Measurement of hepatic Ra UDP-glucose in vivo in rats: relation to glycogen deposition and labeling patterns. Am J Physiol 272:E155-62
Witkowska, H E; Green, B N; Carlquist, M et al. (1996) Intact noncovalent dimer of estrogen receptor ligand-binding domain can be detected by electrospray ionization mass spectrometry. Steroids 61:433-8
Chantilis, S; Dombroski, R; Shackleton, C H et al. (1996) Metabolism of 5 alpha-dihydroprogesterone in women and men: 3 beta- and 3 alpha-,6 alpha-dihydroxy-5 alpha-pregnan-20-ones are major urinary metabolites. J Clin Endocrinol Metab 81:3644-9
Carr, A C; Winterbourn, C C; van den Berg, J J (1996) Peroxidase-mediated bromination of unsaturated fatty acids to form bromohydrins. Arch Biochem Biophys 327:227-33
Palermo, M; Gomez-Sanchez, C; Roitman, E et al. (1996) Quantitation of cortisol and related 3-oxo-4-ene steroids in urine using gas chromatography/mass spectrometry with stable isotope-labeled internal standards. Steroids 61:583-9

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