Abstract

The goal of this research is to study the molecular mechanisms involved in controlling testicular physiology and male reproduction.
Aim 1 will investigate the control of transcription of the rat inhibin a-subunit gene by GATA-binding proteins in MA-10 cells and rat Sertoli and Leydig cells, and will identify cofactors and cell-specific proteins that interact with GATA-1 to regulate the a-subunit gene.
Aim 2 is to investigate the regulation of two promoters within the rat bB-subunit gene that give rise to two species (4.8- and 3.7-kb) of the bB-subunit mRNA from alternate initiation sites.
Aim 3 will investigate the developmental expression and hormonal regulation of GATA-binding proteins in the rat testis to determine whether any observed changes correlate to the known regulation of the a- and bB-subunit genes.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK034449-10
Application #
6176354
Study Section
Biochemical Endocrinology Study Section (BCE)
Program Officer
Sato, Sheryl M
Project Start
1985-09-01
Project End
2001-06-30
Budget Start
2000-07-01
Budget End
2001-06-30
Support Year
10
Fiscal Year
2000
Total Cost
$173,520
Indirect Cost

  Institution

Name
Population Council
Department
Type
DUNS #
071050090
City
New York
State
NY
Country
United States
Zip Code
10017

  Publications

Zhang, Zhifang; Wu, Ai Zhen; Feng, Zong-Ming et al. (2002) Gonadotropins, via cAMP, negatively regulate GATA-1 gene expression in testicular cells. Endocrinology 143:829-36
Feng, Z M; Wu, A Z; Zhang, Z et al. (2000) GATA-1 and GATA-4 transactivate inhibin/activin beta-B-subunit gene transcription in testicular cells. Mol Endocrinol 14:1820-35
Feng, Z M; Wu, A Z; Chen, C L (1998) Testicular GATA-1 factor up-regulates the promoter activity of rat inhibin alpha-subunit gene in MA-10 Leydig tumor cells. Mol Endocrinol 12:378-90
Feng, Z M; Wu, A Z; Chen, C L (1995) Characterization and regulation of two testicular inhibin/activin beta B-subunit messenger ribonucleic acids that are transcribed from alternate initiation sites. Endocrinology 136:947-55
Feng, Z M; Chen, C L (1994) Negative control of the rat inhibin alpha subunit promoter in MA-10 Leydig tumour cells. J Mol Endocrinol 13:39-47
Feng, Z M; Madigan, M B; Chen, C L (1993) Expression of type II activin receptor genes in the male and female reproductive tissues of the rat. Endocrinology 132:2593-600
Chen, C L; Pignataro, O P; Feng, Z M (1993) Inhibin/activin subunits and activin receptor are co-expressed in Leydig tumor cells. Mol Cell Endocrinol 94:137-43
Lopez-Calderon, A; Ariznavarreta, C; Chen, C L (1991) Influence of chronic restraint stress on pro-opiomelanocortin mRNA and beta-endorphin in the rat hypothalamus. J Mol Endocrinol 7:197-204
Voglmayr, J K; Mizumachi, M; Washington, D W et al. (1990) Immunization of rams against human recombinant inhibin alpha-subunit delays, augments, and extends season-related increase in blood gonadotropin levels. Biol Reprod 42:81-6
Feng, Z M; Bardin, C W; Chen, C L (1989) Characterization and regulation of testicular inhibin beta-subunit mRNA. Mol Endocrinol 3:939-48

Showing the most recent 10 out of 16 publications