The goal of this research proposal is to study the immunobiology of a mouse-mesangial cell derived factor which has immunological properties. Thus, studies will be done as follows: (a) based on preliminary observations in our laboratory, we have identified a mouse mesangial cell derived immunoregulatory factor which stimulates lymphocyte proliferation by its action on the monocyte/macrophage; also it has been shown that this factor stimulates spleen monocyte/macrophage replication. Further studies are proposed to examine the effect of this factor on monocyte/macrophage function as they relate to inflammation--thus does it stimulate monocyte Interleuken-I release, Ia antigen expression, and how does it modulate monocyte/macrophage production of fibronectin, C5, plasminogen activator production, production of the metabolites of arachidonic acid metabolism, and the expression of Fc receptor activity? Does it posess chemotactant activity for monocytes/macrophages? Studies will also be done to isolate and biochemically characterize the mediators responsible. The information obtained will be fundamental to our knowledge of: (1) how mesangial cells may provide a feedback regulatory loop which modifies the physiology of infiltrating monocytes/macrophages during nephritis; (2) provide a model to study the nature of immunoregulation at a local (tissue specific) level which may have relevance to other areas of cell pathology; (3) identification of a substance which may eventually be used as an immunotherapeutic substance.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK034628-03
Application #
3232914
Study Section
Immunological Sciences Study Section (IMS)
Project Start
1984-12-01
Project End
1987-11-30
Budget Start
1986-12-01
Budget End
1987-11-30
Support Year
3
Fiscal Year
1987
Total Cost
Indirect Cost
Name
University of Cincinnati
Department
Type
Schools of Medicine
DUNS #
City
Cincinnati
State
OH
Country
United States
Zip Code
45221