Abstract

This is a proposal to elucidate the role of 5HBG and its plasma membrane receptor in androgen (A) and estrogen (E) regulation of the human prostate.
Aim 1 is to clone and characterize the cDNA encoding the SHBG-R. The receptor will be cloned from human prostate expression libraries.
Aim two will determine the effects of SHBG-R on prostatic growth. An immortalized human prostate stromal cell line will be prepared for these studies. The effects of purified SHBG and sex steroids on stromal cell growth in vitro will be determined together with the relationship between the 5HBG-R and cAMP response element-dependent gene regulation.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK036714-09
Application #
2654496
Study Section
Biochemical Endocrinology Study Section (BCE)
Program Officer
Margolis, Ronald N
Project Start
1986-01-01
Project End
2000-01-31
Budget Start
1998-02-01
Budget End
1999-01-31
Support Year
9
Fiscal Year
1998
Total Cost
Indirect Cost

  Institution

Name
St. Luke's-Roosevelt Institute for Health Sciences
Department
Type
DUNS #
City
New York
State
NY
Country
United States
Zip Code
10019

  Publications

Rosner, W; Hryb, D J; Khan, M S et al. (1999) Sex hormone-binding globulin mediates steroid hormone signal transduction at the plasma membrane. J Steroid Biochem Mol Biol 69:481-5
Nakhla, A M; Leonard, J; Hryb, D J et al. (1999) Sex hormone-binding globulin receptor signal transduction proceeds via a G protein. Steroids 64:213-6
Rosner, W; Hryb, D J; Khan, M S et al. (1998) Androgens, estrogens, and second messengers. Steroids 63:278-81
Nakhla, A M; Romas, N A; Rosner, W (1997) Estradiol activates the prostate androgen receptor and prostate-specific antigen secretion through the intermediacy of sex hormone-binding globulin. J Biol Chem 272:6838-41
Nakhla, A M; Rosner, W (1996) Stimulation of prostate cancer growth by androgens and estrogens through the intermediacy of sex hormone-binding globulin. Endocrinology 137:4126-9
Blithe, D L; Khan, M S; Rosner, W (1992) Comparison of the carbohydrate composition of rat and human corticosteroid-binding globulin: species specific glycosylation. J Steroid Biochem Mol Biol 42:475-8
Rosner, W; Hryb, D J; Khan, M S et al. (1992) Sex hormone-binding globulin. Binding to cell membranes and generation of a second messenger. J Androl 13:101-6
Rosner, W; Hryb, D J; Khan, M S et al. (1991) Sex hormone-binding globulin: anatomy and physiology of a new regulatory system. J Steroid Biochem Mol Biol 40:813-20
Khan, M S; Hryb, D J; Hashim, G A et al. (1990) Delineation and synthesis of the membrane receptor-binding domain of sex hormone-binding globulin. J Biol Chem 265:18362-5
Rosner, W (1990) The functions of corticosteroid-binding globulin and sex hormone-binding globulin: recent advances. Endocr Rev 11:80-91

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