Abstract

Using the techniques of molecular cloning, we propose to isolate and characterize plasmids harboring sequences complementary to proline-rich protein messenger RNA induced in the parotid glands of rats following injection of animals with the beta-adrenergic drug isoproterenol. The plasmids will be employed as hybridization probes for the identification of genomic clones containing the structural information for proline-rich proteins. The genomic clones and the cDNA plasmid clones will be studied structurally by the techniques of restriction endonuclease site mapping and DNA sequencing. Both types of clones will be employed to investigate the mode of action of isoproterenol in a variety of mammalian tissues.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
3R01DK036812-01S1
Application #
3235325
Study Section
Pathobiochemistry Study Section (PBC)
Project Start
1985-06-01
Project End
1986-06-30
Budget Start
1986-01-01
Budget End
1986-06-30
Support Year
1
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
University of California Davis
Department
Type
Earth Sciences/Resources
DUNS #
094878337
City
Davis
State
CA
Country
United States
Zip Code
95618

  Publications

Waters, C A; Morand, J N; Schatzman, R C et al. (1998) Induction of p34cdc2 in mouse parotid glands upon activation of beta1-adrenergic receptors. Cell Mol Biol (Noisy-le-grand) 44:333-42
Miao, Y J; Subramaniam, N; Carlson, D M (1995) cDNA cloning and characterization of rat salivary glycoproteins. Novel members of the proline-rich-protein multigene families. Eur J Biochem 228:343-50
Carlson, D M (1993) Salivary proline-rich proteins: biochemistry, molecular biology, and regulation of expression. Crit Rev Oral Biol Med 4:495-502
Lin, H H; Ann, D K (1991) Molecular characterization of rat multigene family encoding proline-rich proteins. Genomics 10:102-13
Carlson, D M (1991) Glycoproteins: carbohydrates to cloning. Glycobiology 1:463-7
Carlson, D M; Zhou, J; Wright, P S (1991) Molecular structure and transcriptional regulation of the salivary gland proline-rich protein multigene families. Prog Nucleic Acid Res Mol Biol 41:1-22
Gadbois, D M; Salo, W L; Ann, D K et al. (1988) The preparation of poly(A)+mRNA from the hagfish slime gland. Prep Biochem 18:67-76
Ann, D K; Wu, M M; Huang, T et al. (1988) Retinol-regulated gene expression in human tracheobronchial epithelial cells. Enhanced expression of elongation factor EF-1 alpha. J Biol Chem 263:3546-9
Nichols, R; Carlson, D M; Dixon, J E (1987) Characterization of primary translation products from ovine and rat salivary gland mRNAs. Biochem Biophys Res Commun 149:244-8
Mehansho, H; Ann, D K; Butler, L G et al. (1987) Induction of proline-rich proteins in hamster salivary glands by isoproterenol treatment and an unusual growth inhibition by tannins. J Biol Chem 262:12344-50