Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK037255-11
Application #
2140040
Study Section
Metallobiochemistry Study Section (BMT)
Project Start
1985-08-01
Project End
1999-07-31
Budget Start
1996-08-01
Budget End
1997-07-31
Support Year
11
Fiscal Year
1996
Total Cost
Indirect Cost
Name
Virginia Polytechnic Institute and State University
Department
Biochemistry
Type
Schools of Earth Sciences/Natur
DUNS #
003137015
City
Blacksburg
State
VA
Country
United States
Zip Code
24061
Fisher, Karl; Lowe, David J; Tavares, Pedro et al. (2007) Conformations generated during turnover of the Azotobacter vinelandii nitrogenase MoFe protein and their relationship to physiological function. J Inorg Biochem 101:1649-56
Xiao, Yuming; Fisher, Karl; Smith, Matt C et al. (2006) How nitrogenase shakes--initial information about P-cluster and FeMo-cofactor normal modes from nuclear resonance vibrational spectroscopy (NRVS). J Am Chem Soc 128:7608-12
Durrant, Marcus C; Francis, Amanda; Lowe, David J et al. (2006) Evidence for a dynamic role for homocitrate during nitrogen fixation: the effect of substitution at the alpha-Lys426 position in MoFe-protein of Azotobacter vinelandii. Biochem J 397:261-70
Fisher, Karl; Dilworth, Michael J; Newton, William E (2006) Azotobacter vinelandii vanadium nitrogenase: formaldehyde is a product of catalyzed HCN reduction, and excess ammonia arises directly from catalyzed azide reduction. Biochemistry 45:4190-8
Maskos, Zofia; Fisher, Karl; Sorlie, Morten et al. (2005) Variant MoFe proteins of Azotobacter vinelandii: effects of carbon monoxide on electron paramagnetic resonance spectra generated during enzyme turnover. J Biol Inorg Chem 10:394-406
Fisher, Karl; Newton, William E (2005) Nitrogenase proteins from Gluconacetobacter diazotrophicus, a sugarcane-colonizing bacterium. Biochim Biophys Acta 1750:154-65
Han, Jaehong; Newton, William E (2004) Differentiation of acetylene-reduction sites by stereoselective proton addition during Azotobacter vinelandii nitrogenase-catalyzed C2D2 reduction. Biochemistry 43:2947-56
Fisher, K; Newton, W E; Lowe, D J (2001) Electron paramagnetic resonance analysis of different Azotobacter vinelandii nitrogenase MoFe-protein conformations generated during enzyme turnover: evidence for S = 3/2 spin states from reduced MoFe-protein intermediates. Biochemistry 40:3333-9
Fisher, K; Dilworth, M J; Kim, C H et al. (2000) Azotobacter vinelandii nitrogenases containing altered MoFe proteins with substitutions in the FeMo-cofactor environment: effects on the catalyzed reduction of acetylene and ethylene. Biochemistry 39:2970-9
Fisher, K; Dilworth, M J; Kim, C H et al. (2000) Azotobacter vinelandii nitrogenases with substitutions in the FeMo-cofactor environment of the MoFe protein: effects of acetylene or ethylene on interactions with H+, HCN, and CN-. Biochemistry 39:10855-65

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