Abstract

The long term goal of this research is to understand the structure and function of androgen receptors in the male hormone sensitive organs and cells.
The specific aims i n the next several years are: I. Preparation of specific anti-androgen receptor antibodies by lymphocyte transformation and hybridoma technology and/or by epitope selection methods. II. Cloning of androgen receptor cDNA by using expression libraries and antibody screening techniques, structural analysis of cDNA, and study of the regulation of the expression of androgen receptor gene in target organs. III. Cellular and intracellular localization, and structural analysis of androgen receptors by immunochemical analysis. Monoclonal antibodies and cDNA for androgen receptors may be used in the characterization of androgen receptors and its regulation in the abnormal organs and in the study of their roles in causing or in the progression of diseases such as benign prostate hyperplasia or prostate cancers.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK037694-03
Application #
3236733
Study Section
(SSS)
Project Start
1986-07-01
Project End
1991-06-30
Budget Start
1988-07-01
Budget End
1989-06-30
Support Year
3
Fiscal Year
1988
Total Cost
Indirect Cost

  Institution

Name
University of Chicago
Department
Type
Schools of Medicine
DUNS #
225410919
City
Chicago
State
IL
Country
United States
Zip Code
60637

  Publications

Kokontis, J; Takakura, K; Hay, N et al. (1994) Increased androgen receptor activity and altered c-myc expression in prostate cancer cells after long-term androgen deprivation. Cancer Res 54:1566-73
Liao, S (1994) Androgen action: molecular mechanism and medical application. J Formos Med Assoc 93:741-51
Song, C; Kokontis, J M; Hiipakka, R A et al. (1994) Ubiquitous receptor: a receptor that modulates gene activation by retinoic acid and thyroid hormone receptors. Proc Natl Acad Sci U S A 91:10809-13
Nakada, S Y; di Sant'Agnese, P A; Moynes, R A et al. (1993) The androgen receptor status of neuroendocrine cells in human benign and malignant prostatic tissue. Cancer Res 53:1967-70
Horie, K; Takakura, K; Imai, K et al. (1992) Immunohistochemical localization of androgen receptor in the human endometrium, decidua, placenta and pathological conditions of the endometrium. Hum Reprod 7:1461-6
Horie, K; Takakura, K; Fujiwara, H et al. (1992) Immunohistochemical localization of androgen receptor in the human ovary throughout the menstrual cycle in relation to oestrogen and progesterone receptor expression. Hum Reprod 7:184-90
Trifiro, M; Gottlieb, B; Pinsky, L et al. (1991) The 56/58 kDa androgen-binding protein in male genital skin fibroblasts with a deleted androgen receptor gene. Mol Cell Endocrinol 75:37-47
Kokontis, J; Ito, K; Hiipakka, R A et al. (1991) Expression and function of normal and LNCaP androgen receptors in androgen-insensitive human prostatic cancer cells. Altered hormone and antihormone specificity in gene transactivation. Receptor 1:271-9
Ris-Stalpers, C; Trifiro, M A; Kuiper, G G et al. (1991) Substitution of aspartic acid-686 by histidine or asparagine in the human androgen receptor leads to a functionally inactive protein with altered hormone-binding characteristics. Mol Endocrinol 5:1562-9
Kokontis, J; Liao, S; Chang, C (1991) Transcriptional activation by TR3 receptor, a member of the steroid receptor superfamily. Receptor 1:261-70

Showing the most recent 10 out of 25 publications