Abstract

To understand the underlying mechanisms of regulating prostatic growth, we have focused our attention on the stromal-epithelial interaction. The following goals have been accomplished: 1) Rat prostatic tissues have been dissociated into single cell suspensions. Pure epithelial cells were isolated and characterized. Morphological and biochemical characterizations of stromal cells are presently under investigation. 2) Several biochemical methodologies were developed to assess the responsiveness of epithelial cells to stromal induction. These include steroid metabolism, androgen receptor assay, 2-dimensional polyacrylamide gel electrophoresis and enzyme activity assay. These methodologies have been applied to analyze the stromal-epithelial interaction in the developing prostate. 3) Human BPH tissues were successfully grown in nude mice. The influence of embryonic mesenchyme on human BPH growth and development is under investigation. 4) To evaluate the inductive potential of adult prostate to embryonic epithelium isolated from either urogenital sinus or bladder, a recombinant experiment was developed. Results indicate that adult prostate (stroma?) positively programmed the development of embryonic epithelium to prostate gland.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
7R01DK038649-01
Application #
3238074
Study Section
Pathology B Study Section (PTHB)
Project Start
1986-07-01
Project End
1988-11-30
Budget Start
1986-07-01
Budget End
1988-11-30
Support Year
1
Fiscal Year
1986
Total Cost
Indirect Cost

  Institution

Name
University of Texas MD Anderson Cancer Center
Department
Type
Hospitals
DUNS #
001910777
City
Houston
State
TX
Country
United States
Zip Code
77030

  Publications

Thalmann, G N; Sikes, R A; Chang, S M et al. (1996) Suramin-induced decrease in prostate-specific antigen expression with no effect on tumor growth in the LNCaP model of human prostate cancer. J Natl Cancer Inst 88:794-801
Marengo, S R; Chung, L W (1994) An orthotopic model for the study of growth factors in the ventral prostate of the rat: effects of epidermal growth factor and basic fibroblast growth factor. J Androl 15:277-86
Wu, H C; Hsieh, J T; Gleave, M E et al. (1994) Derivation of androgen-independent human LNCaP prostatic cancer cell sublines: role of bone stromal cells. Int J Cancer 57:406-12
Thalmann, G N; Anezinis, P E; Chang, S M et al. (1994) Androgen-independent cancer progression and bone metastasis in the LNCaP model of human prostate cancer. Cancer Res 54:2577-81
Zhau, H E; Pisters, L L; Hall, M C et al. (1994) Biomarkers associated with prostate cancer progression. J Cell Biochem Suppl 19:208-16
Zhau, H E; Hong, S J; Chung, L W (1994) A fetal rat urogenital sinus mesenchymal cell line (rUGM): accelerated growth and conferral of androgen-induced growth responsiveness upon a human bladder cancer epithelial cell line in vivo. Int J Cancer 56:706-14
Hoosein, N M; Logothetis, C J; Chung, L W (1993) Differential effects of peptide hormones bombesin, vasoactive intestinal polypeptide and somatostatin analog RC-160 on the invasive capacity of human prostatic carcinoma cells. J Urol 149:1209-13
Gleave, M E; Hsieh, J T; Wu, H C et al. (1993) Epidermal growth factor receptor-mediated autocrine and paracrine stimulation of human transitional cell carcinoma. Cancer Res 53:5300-7
Chung, L W (1993) Implications of stromal-epithelial interaction in human prostate cancer growth, progression and differentiation. Semin Cancer Biol 4:183-92
McDonnell, T J; Troncoso, P; Brisbay, S M et al. (1992) Expression of the protooncogene bcl-2 in the prostate and its association with emergence of androgen-independent prostate cancer. Cancer Res 52:6940-4

Showing the most recent 10 out of 23 publications