Abstract

By using all available electrophysiological techniques, including tight-seal patch-clamp methods, we will attempt to secure the basic information concerning the characteristics of the ionic channels in the smooth muscles of the normal urinary tract. This will be a multilevel study, ranging from tissue phenomena, through individual dispersed cells to single-channel events. We believe that such fundamental knowledge serves as an essential basis for understanding pathophysiological derangements in disease states. The properties to be learned include the nature of the charge-carriers, kinetics of activation, inactivation, deactivation, and reactivation of various conductances, reversal potentials, and pharmacological responses. Differences in the properties of ionic channels in different parts of the urinary tract will be examined to provide a basis of understanding the varied functions of the different parts. Also species differences will be explored in search for the optimum animal model for human urinary tract tissues. The actions of cholinergic, adrenergic, purinergic, and peptide neurotransmitters, as well as those of calcium-channel effectors will be studied at all the levels of cellular organization mentioned.
A specific aim i s to obtain information applicable to clinical therapeutic uses.

  Funding Agency

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK039731-05
Application #
2141023
Study Section
Diabetes, Endocrinology and Metabolic Diseases B Subcommittee (DDK)
Project Start
1987-09-30
Project End
1995-08-31
Budget Start
1991-09-01
Budget End
1995-08-31
Support Year
5
Fiscal Year
1991
Total Cost
Indirect Cost

  Institution

Name
Suny Downstate Medical Center
Department
Pharmacology
Type
Schools of Medicine
DUNS #
068552207
City
Brooklyn
State
NY
Country
United States
Zip Code
11203