Studies on mechanisms of action of hormones are performed in the least ambiguous fashion when the environment of the target cell can be closely controlled, and when cause-and-effect (not simply correlative) relationships can be established. Previous work in this laboratory on hamster yolk-sac erythroid cells has shown that these cells: undergo nearly the entire spectrum of globin ontogeny in the embryonic circulation; demonstrate globin ontogeny when grown in culture if both erythropoietin and triiodothyronine are present in the culture medium; and possess plasma-membrane receptors specific for erythropoietin and nuclear receptors specific for triiodothyronine. We now propose experiments designed to investigate responses of hamster yolk-sac erythroid cells to these hormones, singly and together. The experimental approach will include attempts to isolate the hormone-specific receptors and attempts to quantify receptor- specific mRNA.
| Boussios, T; Lee, K M; Li, H et al. (1993) Expression of adult globin mRNAs in hamster yolk-sac erythroid cells during ontogeny. Exp Hematol 21:901-6 |
| Li, H; Subar, M; Lee, K M et al. (1992) Cloning and sequence analysis of two embryonic beta-like globin cDNAs (y and z) of hamster. Biochim Biophys Acta 1130:218-20 |
| Lee, K M; Subar, M; Li, H et al. (1992) Cloning of two adult hamster globin cDNAs (alpha and beta major). Biochim Biophys Acta 1130:343-4 |
| Lee, K M; Bertles, J F; Boussios, T (1991) Translational control of globin chain ontogeny in hamster yolk sac erythroid cells. J Biol Chem 266:20555-60 |
| Boussios, T; Bertles, J F; Goldwasser, E (1989) Erythropoietin. Receptor characteristics during the ontogeny of hamster yolk sac erythroid cells. J Biol Chem 264:16017-21 |
