The applicant and his co-investigator, Dr. Gerry Cunha, have focused their studies on the cellular and molecular mechanisms of estrogen action on developing and adult prostate with an emphasis on the role of tissue age on cell-cell interactions, estrogen receptors, growth factor pathways. The hypothesis suggested states that estrogens and androgens and the developmental control of prostatic cell growth may be regulated by cell-cell interactions mediated by the local production and action of peptide growth factors. In the first aim, Dr. Dahiya proposes to look at the estrogenic effects on prostate of the neonate and adult mouse using both wild-type and estrogen receptor knock-out mice. Next he will categorize growth factor and receptors during estrogen induced prostatic squamous metaplasia. The role of mesenchymal-epithelial interactions in the estrogenic response of the prostate will be examined. Finally, the applicant will analyze cellular and molecular pathways of estrogen action in neonatal prostates.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK047517-07
Application #
6177041
Study Section
Reproductive Endocrinology Study Section (REN)
Program Officer
Mullins, Christopher V
Project Start
1993-09-30
Project End
2002-06-30
Budget Start
2000-07-01
Budget End
2001-06-30
Support Year
7
Fiscal Year
2000
Total Cost
$263,226
Indirect Cost
Name
University of California San Francisco
Department
Urology
Type
Schools of Medicine
DUNS #
094878337
City
San Francisco
State
CA
Country
United States
Zip Code
94143
Shiina, Hiroaki; Igawa, Mikio; Breault, Julia et al. (2003) The human T-cell factor-4 gene splicing isoforms, Wnt signal pathway, and apoptosis in renal cell carcinoma. Clin Cancer Res 9:2121-32
Marker, Paul C; Dahiya, Rajvir; Cunha, Gerald R (2003) Spontaneous mutation in mice provides new insight into the genetic mechanisms that pattern the seminal vesicles and prostate gland. Dev Dyn 226:643-53
Marker, Paul C; Donjacour, Annemarie A; Dahiya, Rajvir et al. (2003) Hormonal, cellular, and molecular control of prostatic development. Dev Biol 253:165-74
Patra, Samir K; Patra, Aditi; Zhao, Hong et al. (2003) Methyl-CpG-DNA binding proteins in human prostate cancer: expression of CXXC sequence containing MBD1 and repression of MBD2 and MeCP2. Biochem Biophys Res Commun 302:759-66
Cunha, Gerald R; Hayward, Simon W; Wang, Y Z et al. (2003) Role of the stromal microenvironment in carcinogenesis of the prostate. Int J Cancer 107:1-10
Staack, Andrea; Kassis, Adrienne P; Olshen, Adam et al. (2003) Quantitation of apoptotic activity following castration in human prostatic tissue in vivo. Prostate 54:212-9
Tanji, N; Satoh, H; Takagi-Morishita, Y et al. (2003) Induction of apoptosis by castration in epithelium of the mouse seminal vesicles. Arch Androl 49:409-15
Li, Long-Cheng; Zhao, Hong; Shiina, Hiroaki et al. (2003) PGDB: a curated and integrated database of genes related to the prostate. Nucleic Acids Res 31:291-3
Donjacour, Annemarie A; Thomson, Axel A; Cunha, Gerald R (2003) FGF-10 plays an essential role in the growth of the fetal prostate. Dev Biol 261:39-54
Thomson, Axel A; Timms, Barry G; Barton, Lesley et al. (2002) The role of smooth muscle in regulating prostatic induction. Development 129:1905-12

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