Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK050080-02
Application #
2151077
Study Section
Biochemical Endocrinology Study Section (BCE)
Project Start
1995-08-01
Project End
1999-06-30
Budget Start
1996-07-01
Budget End
1997-06-30
Support Year
2
Fiscal Year
1996
Total Cost
Indirect Cost
Name
University of Illinois Urbana-Champaign
Department
Biochemistry
Type
Schools of Arts and Sciences
DUNS #
041544081
City
Champaign
State
IL
Country
United States
Zip Code
61820
Mao, Chengjian; Flavin, Kathryn Goolsby; Wang, Stanley et al. (2006) Analysis of RNA-protein interactions by a microplate-based fluorescence anisotropy assay. Anal Biochem 350:222-32
Wang, Stanley Y; Ahn, Bonnie S; Harris, Rebecca et al. (2004) Fluorescence anisotropy microplate assay for analysis of steroid receptor-DNA interactions. Biotechniques 37:807-8, 810-7
Goolsby, Kathryn M; Shapiro, David J (2003) RNAi-mediated depletion of the 15 KH domain protein, vigilin, induces death of dividing and non-dividing human cells but does not initially inhibit protein synthesis. Nucleic Acids Res 31:5644-53
Dodson, Robin E; Goolsby, Kathryn M; Acena-Nagel, Maria et al. (2003) RNA gel shift assays for analysis of hormone control of mRNA stability. Methods Enzymol 364:350-61
Dodson, Robin E; Shapiro, David J (2002) Regulation of pathways of mRNA destabilization and stabilization. Prog Nucleic Acid Res Mol Biol 72:129-64
Kanamori, H; Shapiro, D J (1999) Differential display combined with a regulated transient expression system. Biotechniques 26:1018-20
Zhang, C C; Krieg, S; Shapiro, D J (1999) HMG-1 stimulates estrogen response element binding by estrogen receptor from stably transfected HeLa cells. Mol Endocrinol 13:632-43
Kanamori, H; Dodson, R E; Shapiro, D J (1998) In vitro genetic analysis of the RNA binding site of vigilin, a multi-KH-domain protein. Mol Cell Biol 18:3991-4003
Dodson, R E; Shapiro, D J (1997) Vigilin, a ubiquitous protein with 14 K homology domains, is the estrogen-inducible vitellogenin mRNA 3'-untranslated region-binding protein. J Biol Chem 272:12249-52