The mechanisms controlling human placental GH and CS gene activation and repression in placenta will be investigated. In the proposed studies, the following aims will be pursued: 1) the individual enhansons characterizing the enhancer will be investigated; 2) multiple enhancer interactions will be characterized in placental and pituitary cells; 3) the relationship of the enhancer interactions with pituitary-specific P element repressors will be investigated; 4) the chromatin structure and matrix attachment regions in the CS/GH locus will be defined; and 5) enhancer (CSEF-1) and inhibitor (CSInrF) proteins will be isolated, sequenced, and cloned.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK051492-04
Application #
2905880
Study Section
Biochemical Endocrinology Study Section (BCE)
Program Officer
Sato, Sheryl M
Project Start
1996-09-30
Project End
2000-08-31
Budget Start
1999-09-01
Budget End
2000-08-31
Support Year
4
Fiscal Year
1999
Total Cost
Indirect Cost
Name
Mayo Clinic, Rochester
Department
Type
DUNS #
City
Rochester
State
MN
Country
United States
Zip Code
55905