Recent studies have indicated that cholesterol is essential for normal brain development and that the central nervous system depends mainly on de novo cholesterol biosynthesis. We have shown that peroxisomes are essential for cholesterol biosynthesis, due to the fact that the entire pathway for the biosynthesis of farnesyl diphosphate (FPP) from mevalonate is exclusively found in peroxisomes. Therefore, peroxisomes must play a critical functional role in this process in the CNS. However, no information is available on the peroxisomal isoprenoid/cholesterol biosynthesis pathway in normal brain or on the compartmentalization of isoprene metabolism in the CNS. An animal model for Zellweger syndrome (i.e., a peroxisomal PEX2 knockout mouse) has been recently developed. These mice provide an important model to study the role of peroxisomal function in the CNS in the pathogenesis of the neurological abnormalities observed in these diseases. In this proposal we address the hypothesis that peroxisomes have a central role in isoprenoid/cholesterol biosynthesis in the CNS and that in PEX2 knockout mice, the isoprenoid/cholesterol biosynthesis in the CNS is significantly altered. To test this hypothesis the following specific aims are proposed: 1) To determine the role of peroxisomes in isoprenoid/cholesterol biosynthesis in normal neonatal mouse brain. The isoprenoid biosynthetic pathway in neonatal mouse brain will be analyzed by subcellular fractionation, activity and immunoblot analysis of the gradient fractions, and immunohistochemistry and in situ hybridization techniques. We will also determine if the relative distribution of peroxisomal and ER contribution to sterol/non-sterol biosynthesis changes during development. 2) To test for defects in lipid composition and cholesterol/dolichol metabolism in PEX2 deficient mice. We will determine how the regulation of cholesterol enzymes and the rate limiting steps in the pathway may be altered by the decompartmentalization of cholesterol synthesis due to the absence of peroxisomes. The studies are designed to ascertain whether there is a basis to implicate cholesterol in some of the neurologic defects observed in the Zellweger mouse. Thus a comparison of isoprenoid/cholesterol metabolism in the PEX2 deficient and normal animals can render insight into the mechanism(s) for the neurological phenotype seen in the knockout mice.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
3R01DK058238-03S1
Application #
6743586
Study Section
Medical Biochemistry Study Section (MEDB)
Program Officer
Mckeon, Catherine T
Project Start
2001-05-01
Project End
2005-04-30
Budget Start
2003-05-01
Budget End
2004-04-30
Support Year
3
Fiscal Year
2003
Total Cost
$44,001
Indirect Cost
Name
San Diego State University
Department
Biology
Type
Schools of Arts and Sciences
DUNS #
073371346
City
San Diego
State
CA
Country
United States
Zip Code
92182
Kovacs, Werner J; Charles, Khanichi N; Walter, Katharina M et al. (2012) Peroxisome deficiency-induced ER stress and SREBP-2 pathway activation in the liver of newborn PEX2 knock-out mice. Biochim Biophys Acta 1821:895-907
Kovacs, Werner J; Tape, Khanichi N; Shackelford, Janis E et al. (2009) Peroxisome deficiency causes a complex phenotype because of hepatic SREBP/Insig dysregulation associated with endoplasmic reticulum stress. J Biol Chem 284:7232-45
Clizbe, Daun B; Owens, Michelle L; Masuda, Kimberly R et al. (2007) IDI2, a second isopentenyl diphosphate isomerase in mammals. J Biol Chem 282:6668-76
Kovacs, Werner J; Tape, Khanichi N; Shackelford, Janis E et al. (2007) Localization of the pre-squalene segment of the isoprenoid biosynthetic pathway in mammalian peroxisomes. Histochem Cell Biol 127:273-90
Kovacs, Werner J; Shackelford, Janis E; Tape, Khanichi N et al. (2004) Disturbed cholesterol homeostasis in a peroxisome-deficient PEX2 knockout mouse model. Mol Cell Biol 24:1-13
Breitling, Rainer (2004) Pathogenesis of peroxisomal deficiency disorders (Zellweger syndrome) may be mediated by misregulation of the GABAergic system via the diazepam binding inhibitor. BMC Pediatr 4:5
Breitling, Rainer; Laubner, Daniela; Clizbe, Daun et al. (2003) Isopentenyl-diphosphate isomerases in human and mouse: evolutionary analysis of a mammalian gene duplication. J Mol Evol 57:282-91
Kovacs, Werner J; Krisans, Skaidrite (2003) Cholesterol biosynthesis and regulation: role of peroxisomes. Adv Exp Med Biol 544:315-27
Lewin, Tal M; Van Horn, Cynthia G; Krisans, Skaidrite K et al. (2002) Rat liver acyl-CoA synthetase 4 is a peripheral-membrane protein located in two distinct subcellular organelles, peroxisomes, and mitochondrial-associated membrane. Arch Biochem Biophys 404:263-70
Breitling, Rainer; Sharif, Orzala; Hartman, Michelle L et al. (2002) Loss of compartmentalization causes misregulation of lysine biosynthesis in peroxisome-deficient yeast cells. Eukaryot Cell 1:978-86

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