The goal of this project is to develop a fluorescence-based sensor for glucose that may be used for the continuous monitoring of blood glucose levels in diabetes patients. This project is based on previous observations that the fluorescence of an arylboronic acid may be altered upon formation of a complex with glucose. While the change in fluorescence can be used to determine glucose concentration in solution, it is not specific for glucose as other sugars and related compounds can also induce the fluorescence change. The goal of this project is to develop new methods for building in high selectivity for complex formation with glucose in systems chosen such that the extent of glucose binding can be conveniently determined by fluorescence measurements. This will provide the basis for a practical sensor for the measurement of glucose concentration in the presence of a variety of sugars and other potential interfering substances. The design of specific glucose receptors will be based on computer-aided design of molecular structures that permit the precise positioning of multiple functional groups that will bind to different parts of a glucose molecule. Structures designed by this approach will be synthesized and the binding of glucose and accompanying fluorescence changes will be studied. Initial structures will be further modified to tune the sensor to physiological glucose concentration and other practical issues in glucose sensor development will be addressed.

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
3R01DK059568-02S1
Application #
7004345
Study Section
Special Emphasis Panel (ZRG1)
Program Officer
Arreaza-Rubin, Guillermo
Project Start
2003-04-01
Project End
2006-02-28
Budget Start
2004-03-01
Budget End
2005-02-28
Support Year
2
Fiscal Year
2005
Total Cost
$45,177
Indirect Cost
Name
State University New York Stony Brook
Department
Chemistry
Type
Schools of Arts and Sciences
DUNS #
804878247
City
Stony Brook
State
NY
Country
United States
Zip Code
11794
Ballou, Lisa M; Selinger, Elzbieta S; Choi, Jun Yong et al. (2007) Inhibition of mammalian target of rapamycin signaling by 2-(morpholin-1-yl)pyrimido[2,1-alpha]isoquinolin-4-one. J Biol Chem 282:24463-70