Function of NPHP6, a novel gene for Senior-Loken syndrome (nephronophthisis with retinitis pigmentosa). Senior-Loken syndrome (SLS) is characterized by the association of nephronophthisis and retinitis pigmentosa. Nephronophthisis (NPHP) is an autosomal-recessive cystic kidney disease that constitutes the most frequent genetic cause of chronic renal failure in the first two decades of life. We have previously identified by positional cloning the novel genes (NPHP1 and NPHP4) as mutated in NPHP/SLS types 1 and 4, respectively. The NPHP1 gene product, nephrocystin-1, interacts with signaling proteins that regulate actin organization in the cytoskeleton. We have also recently identified by positional cloning the novel gene (NPHP3), mutations in which cause NPHP/SLS type 3. We showed that mutations in its mouse homolog cause the mouse renal cystic phenotype pcy, which was recently shown to be responsive to therapeutic interventions. In addition, we have recently identified mutations in the human inversin gene (INVS) as causing NPHP type 2. The NPHP gene products are expressed in primary cilia of renal tubule cells and in the connecting cilium of the retina. They are part of a novel protein interaction complex. We also showed that the NPHP genes are conserved in the nematode C. elegans. They are expressed in the same specific cilitated neurons that express gene homologs of the autosomal dominant polycystic kidney disease and Bardet-Biedl syndrome genes. Here, we have identified by positional cloning a novel sixth gene (NPHP6) as causing nephronophthisis (NPHP) with retinitis pigmentosa (i.e., Senior-Loken syndrome; SLS type 6). We detected 8 distinct recessive mutations in 12 SLS families in an anonymous gene KIAAO036 (now termed NPHP6). All mutations were truncation mutations. All patients with NPHP6 mutations had NPHP with retinitis pigmentosa. Mutations in NPHP6 were the most frequent cause of SLS. This proposal is aimed at the genetics of NPHP type 6 and at the functional characterization of the novel NPHP6 gene product """"""""nephrocystin-6"""""""" that we identified. Specifically, we propose to: 1) Identify further mutations in NPHP6 and study their genotype/phenotype relationships. 2) Characterize the function of the NPHP6 gene and study its role in the pathogenesis of nephronophthisis and retinitis pigmentosa. 3) Generate and characterize mouse models of targeted disruption of the Nphp6 gene. Identification of NPHP6 as a new cause of NPHP and SLS opens new inroads into the understanding of disease mechanisms of renal cystic disease and retinitis pigmentosa. ? ?

Agency
National Institute of Health (NIH)
Institute
National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK)
Type
Research Project (R01)
Project #
5R01DK069274-03
Application #
7170039
Study Section
Cellular and Molecular Biology of the Kidney Study Section (CMBK)
Program Officer
Rasooly, Rebekah S
Project Start
2005-03-01
Project End
2009-12-31
Budget Start
2007-01-01
Budget End
2007-12-31
Support Year
3
Fiscal Year
2007
Total Cost
$334,056
Indirect Cost
Name
University of Michigan Ann Arbor
Department
Pediatrics
Type
Schools of Medicine
DUNS #
073133571
City
Ann Arbor
State
MI
Country
United States
Zip Code
48109
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